FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_20.352000000607bc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_20.352000000607bc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24665
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT3341.3541455503750255No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA2991.212244070545307No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA1350.5473342793431989No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA1200.4865193594161768No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA1110.4500304074599635No Hit
GGAGACAAAAAAAAAAAAAAAAAAAAAAAAAAA850.34461787958645856No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA730.29596594364484086No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA680.2756943036691668No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC620.251368335698358No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA620.251368335698358No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA610.24731400770322318No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA550.22298803973241438No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA520.21082505574700994No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA500.2027163997567403No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA500.2027163997567403No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA500.2027163997567403No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA490.19866207176160552No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA490.19866207176160552No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA450.18244475978106628No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC360.14595580782485304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.14595580782485304No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA350.1419014798297182No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA350.1419014798297182No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA340.1378471518345834No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA320.1297384958443138No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA310.125684167849179No Hit
GTGAATGGGAACGTAGGAGACAAAAAAAAAAAA300.1216298398540442No Hit
GTGAATGGGGACGTAGGAGACAAAAAAAAAAAA300.1216298398540442No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC270.10946685586863977No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA270.10946685586863977No Hit
GTGAATGGGGACGTAGGAAAAAAAAAAAAAAAA260.10541252787350497No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA250.10135819987837015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCAGA200.00841291727.0244433
CAACTCA257.5704046E-427.024441
GTATCAT604.0017767E-1127.024441
TCTACGC257.5704046E-427.024443
AACTCAG257.5704046E-427.024442
TATCTAC257.5704046E-427.024441
TATCATC604.0017767E-1127.024442
TACGCAG257.661399E-426.9695135
CTCAGAG200.00849716526.9695134
CTACGCA257.661399E-426.9695134
TACCACT451.5473925E-624.07074727
ATCTACG351.9491492E-423.1638052
ATCATCG652.9758667E-922.8668353
TCATCGC653.0468073E-922.8203584
TCGCAGA603.3323886E-822.4745927
ATCGCAG603.3323886E-822.4745926
CATCGCA707.1704562E-921.1903325
ATACCAC558.982603E-619.65413926
GATACCA707.137906E-515.44253725
TGATACC707.137906E-515.44253724
Produced by FastQC (version 0.11.2)