FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_19.35200000060793.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_19.35200000060793.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24784
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2300.9280180761781794No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC2240.9038089089735314No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA1950.7867979341510652No Hit
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA1650.6657520981278243No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA1230.4962879276952873No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA1200.48418334409296315No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA1170.4720787604906391No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA1150.4640090380890897No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA1100.4438347320852163No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA1040.4196255648805681No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA1020.4115558424790188No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA1010.407520981278244No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA980.39541639767591996No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA900.3631375080697224No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA900.3631375080697224No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA850.34296320206584896No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA830.33489347966429955No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC810.32682375726275015No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA780.31471917366042607No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA730.29454486765655263No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA720.2905100064557779No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA690.2784054228534538No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC690.2784054228534538No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA670.2703357004519044No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA660.26630083925112974No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA630.25419625564880566No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA630.25419625564880566No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA620.250161394448031No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA600.24209167204648158No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA560.22595222724338285No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA550.22191736604260814No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA540.21788250484183347No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA530.21384764364105877No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA510.2057779212395094No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT510.2057779212395094No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA500.20174306003873468No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA490.19770819883795998No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG490.19770819883795998No Hit
CCCCCAATGCGCTCTGAACGTACTCTGCGTTGA490.19770819883795998No Hit
GAACGTACTCTGCGAAAAAAAAAAAAAAAAAAA480.19367333763718528No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA480.19367333763718528No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA460.1856036152356359No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA450.1815687540348612No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA450.1815687540348612No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA450.1815687540348612No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA420.16946417043253711No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT410.16542930923176244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA410.16542930923176244No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.16139444803098774No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA380.15332472562943836No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA370.14928986442866365No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA370.14928986442866365No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA370.14928986442866365No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.14525500322788895No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA340.13718528082633957No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA340.13718528082633957No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA300.12104583602324079No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA290.11701097482246611No Hit
GCTCTGAACGTACTCTGCGTAAAAAAAAAAAAA290.11701097482246611No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTAAAAA290.11701097482246611No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA290.11701097482246611No Hit
GCCCCAATGCGCTCTGAACGTACTCTGCGTTGA280.11297611362169142No Hit
ACGCAGAGTGAATGGGCTCTGAACGTAAAAAAA270.10894125242091673No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA270.10894125242091673No Hit
CAACTCAGAGTGAATGGGCTCTGAACGTACTCT260.10490639122014203No Hit
TATCAACGCAGAGTGAATGGGCTCTTAACGTAC260.10490639122014203No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA260.10490639122014203No Hit
CTGAACGTACTCAAAAAAAAAAAAAAAAAAAAA260.10490639122014203No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA260.10490639122014203No Hit
GAACGTACTCTGCAAAAAAAAAAAAAAAAAAAA250.10087153001936734No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT306.8842324E-527.01
TATCATC351.9617715E-423.1428592
ATCATCG404.8390898E-420.253
TATCAAC1700.019.8529421
GCGTTGA2700.019.027
TCAACGC1850.018.2432423
TCGCAGA450.001069078118.0000027
ATCGCAG450.001069078118.0000026
TCATCGC450.001069078118.0000024
CATCGCA450.001069078118.0000025
ATCAACG1900.017.7631592
CAACGCA1850.016.7837834
CGTTGAT853.746192E-412.70588327
TGCGTTG4100.012.51219526
AACGCAG2600.012.4615385
CTGCGTT4650.011.03225825
TCAATGC1256.876337E-510.88
TAATGCG2007.214112E-910.7999998
AAATGCG1955.9137164E-810.3846168
TCTGCGT8300.09.92168727
Produced by FastQC (version 0.11.2)