FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_18.35200000060779.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_18.35200000060779.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14341
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA2031.4155219301303954No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA1651.15054738163308No Hit
GTATCCAATGCACGTACTCTGCGTTGATACCAC1170.8158426887943657No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA950.6624363712432885No Hit
TATCAACGCAGAGTGAATGGGTATCCAATGCAC640.44627292378495226No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA630.43929990935081237No Hit
GTGAATGGGTATCCAATGCACGTACTCAAAAAA620.43232689491667253No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC590.4114078516142528No Hit
CCAATGCACGTACTCAAAAAAAAAAAAAAAAAA460.32075866397043445No Hit
GTGAATGGGTATCCAATGCACGTACAAAAAAAA460.32075866397043445No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC450.31378564953629456No Hit
GTGAATGGGTATCCAATGAAAAAAAAAAAAAAA440.3068126351021547No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC440.3068126351021547No Hit
GTGAATGGGTATCCAATGCAAAAAAAAAAAAAA410.28589359179973506No Hit
GTGAATGGGTATCCAATGCACGTAAAAAAAAAA400.2789205773655951No Hit
TATCAACGCAGAGTGAATGGGGTATCCAAAAAA400.2789205773655951No Hit
TATCAACGCAGAGTGAATGGGGTATCCAATGCA400.2789205773655951No Hit
GAGTGAATGGGGTATCCAAAAAAAAAAAAAAAA380.2649745484973154No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC380.2649745484973154No Hit
GTGAATGGGGTATCCAATGCACGTACTCAAAAA370.2580015340631755No Hit
GTGAATGGGTATCCAATAAAAAAAAAAAAAAAA370.2580015340631755No Hit
ACGCAGAGTGAATGGGGTATCCAAAAAAAAAAA360.25102851962903566No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC360.25102851962903566No Hit
GTGAATGGGGGTATCCAAAAAAAAAAAAAAAAA340.23708249076075585No Hit
GTGAATGGGGTATCCAATGCACAAAAAAAAAAA320.22313646189247613No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC320.22313646189247613No Hit
ACGCAGAGTGAATGGGTATCCAAAAAAAAAAAA300.20919043302419635No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC280.1952444041559166No Hit
GTATCAACGCAGAGTGAATGGGTATCCAATGCA280.1952444041559166No Hit
ATGGTATCCAATGCACGTACTCTGCGTTGATAC270.1882713897217767No Hit
TATCAACGCAGAGTGAATGGGTATCCAAAAAAA270.1882713897217767No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC260.18129837528763684No Hit
ACGCAGAGTGAATGGGTATCCAATGCACGTACT240.1673523464193571No Hit
ACGCAGAGTGAATGGGGTATCCAATGCACGTAC240.1673523464193571No Hit
GTGAATGGGTATCCAATGCACGAAAAAAAAAAA240.1673523464193571No Hit
GTGAATGGGGTATCCAATGCACGTACAAAAAAA230.16037933198521723No Hit
ACGCAGAGTGAATGGGTATCCAATGCACAAAAA220.15340631755107734No Hit
AACGCAGAGTGAATGGGGTATCCAAAAAAAAAA220.15340631755107734No Hit
GTGGTATCCAATGCACAAAAAAAAAAAAAAAAA210.14643330311693745No Hit
GTGAATGGGGTATCCAATGCACGTAAAAAAAAA200.13946028868279756No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC200.13946028868279756No Hit
GTGGTATCCAATGCACGTACTCTGCGTTGATAC200.13946028868279756No Hit
AACGCAGAGTGAATGGGTATCCAAAAAAAAAAA190.1324872742486577No Hit
GTATCAACGCAGAGTGAATGGGGTATCCAATGC190.1324872742486577No Hit
GTGAATGGGGTATCCAATGCACGTACTCTGCGT190.1324872742486577No Hit
TTGGTATCCAATGCACGTACTCTGCGTTGATAC190.1324872742486577No Hit
CGGTATCCAATGCACGTACTCTGCGTTGATACC180.12551425981451783No Hit
GAGGTATCCAATGCACGTACTCTGCGTTGATAC180.12551425981451783No Hit
GAGTGAATGGGTATCCAAAAAAAAAAAAAAAAA170.11854124538037793No Hit
ATCCAATGCACAAAAAAAAAAAAAAAAAAAAAA170.11854124538037793No Hit
AGGGTATCCAATGCACGTACTCTGCGTTGATAC170.11854124538037793No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.11156823094623806No Hit
AACGCAGAGTGAATGGGGTATCCAATGCACGTA160.11156823094623806No Hit
AACGCAGAGTGAATGGGTATCCAATGCACGTAC160.11156823094623806No Hit
TATCCAATGCACGTACTCTGCGTTGATACCACT150.10459521651209817No Hit
GTGAATGGGGTATCCAATGAAAAAAAAAAAAAA150.10459521651209817No Hit
GTGAATGGGGTATCCAATAAAAAAAAAAAAAAA150.10459521651209817No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA150.10459521651209817No Hit
GTGAATGGGGTATCAAAAAAAAAAAAAAAAAAA150.10459521651209817No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAT350.00527548819.2857157
TGGGGTA955.311449E-1018.4736846
ATGGGGT1001.0550139E-917.5500015
TCAACGC756.62843E-616.1999993
TATCAAC756.62843E-616.1999991
ATCAACG756.62843E-616.1999992
GTTGATA706.859997E-515.42857226
GCGGTAT706.859997E-515.4285727
TTGATAC706.859997E-515.42857227
CGTTGAT706.859997E-515.42857225
GGGGTAT1156.7666406E-915.2608697
TCTGCGT4450.014.86516927
CAACGCA852.1718914E-514.2941184
AACGCAG903.7239446E-513.55
GTGAATG3900.013.1538471
TGAATGG3850.012.9740272
ATGGGTA1704.656613E-1012.7058835
AGGTATC1305.205111E-712.4615398
AATGGGT1757.385097E-1012.3428574
GAATGGG3950.012.3037983
Produced by FastQC (version 0.11.2)