FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_15.3520000006070f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_15.3520000006070f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15123
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAGCTATGCCAACGTACTCTGCGTTGATACCAC1711.1307280301527474No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA820.5422204589036567No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT640.42319645572968323No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT620.40997156648813066No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA560.3702968987634728No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA500.33062223103881505No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA440.2909475633141573No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA420.27772267407260465No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA410.27111022945182833No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA330.21821067248561796No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT320.21159822786484161No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC320.21159822786484161No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA310.20498578324406533No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT290.19176089400251273No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1851484493817364No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA260.1719235601401838No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA260.1719235601401838No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA250.16531111551940753No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA250.16531111551940753No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA220.14547378165707864No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA220.14547378165707864No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT220.14547378165707864No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT210.13886133703630232No Hit
ATGCCAACGTACTCAAAAAAAAAAAAAAAAAAA210.13886133703630232No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA210.13886133703630232No Hit
CCCGTCAGCTATGCCAACGTACTCTGCGTTGAT190.12563644779474972No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA180.11902400317397342No Hit
GTGAATGGGTGTGAGCAACTCTAACTATGCCAA180.11902400317397342No Hit
CCCCTTCAGCTATGCCAACGTACTCTGCGTTGA170.11241155855319712No Hit
ACGCAGAGTGAATGGGTCAGCTATGCCAAAAAA170.11241155855319712No Hit
TATCAACGCAGAGTGAATGGGTCAGCTATGCCA170.11241155855319712No Hit
CCCCCCCCTCAGCTATGCCAACGTACTCTGCGT170.11241155855319712No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA170.11241155855319712No Hit
CCCATCAGCTATGCCAACGTACTCTGCGTTGAT160.10579911393242081No Hit
ACCCTCAGCTATGCCAACGTACTCTGCGTTGAT160.10579911393242081No Hit
CTCCTCAGCTATGCCAACGTACTCTGCGTTGAT160.10579911393242081No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGAT952.1509186E-715.64119627
TCTGCGT4300.015.07903927
GCTCAGC700.001261294613.4635767
ACTCAGC750.002121426912.5660057
CTCTGCG5350.012.11960126
TATCAAC905.792632E-412.0073821
ACTCTGC5700.011.37541625
GCGTTGA1256.42683E-510.80664526
TTCAGCT1256.651087E-510.7708628
TACTCTG6200.010.45804324
CTCAGCT3500.010.3861888
GTACTCT6550.010.31168423
AACGTAC7000.010.0347420
TGCCAAC6950.09.9125716
GCCAACG6950.09.9125717
CAACGTA6950.09.9125719
CCAACGT7050.09.77196618
ACGTACT6950.09.71820521
CGTACTC7000.09.64878922
AGCTATG7200.09.56838311
Produced by FastQC (version 0.11.2)