FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_14.352000000606f1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_14.352000000606f1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17943
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA6023.355068829069832No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC1921.0700551747199465No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA1200.6687844841999665No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA980.5461739954299727No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA970.540600791394973No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA870.4848687510449758No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA760.4235635066599788No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA750.4179903026249791No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA740.4124170985899794No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC730.4068438945549796No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA710.3956974864849802No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA670.3734046703449813No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA650.36225826227498187No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA550.3065262219249847No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA540.30095301788998496No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT520.2898066098199855No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC520.2898066098199855No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA470.2619405896449869No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA470.2619405896449869No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA460.2563673856099872No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA440.24522097753998776No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA440.24522097753998776No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA430.239647773504988No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA420.2340745694699883No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA410.22850136543498856No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA400.2229281613999889No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA400.2229281613999889No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA390.21735495736498914No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA360.20063534525998997No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.20063534525998997No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA360.20063534525998997No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA360.20063534525998997No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT360.20063534525998997No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA350.19506214122499024No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA350.19506214122499024No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT340.18948893718999052No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA320.1783425291199911No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCATA320.1783425291199911No Hit
ATGGGCAACAGAGCAAAAAAAAAAAAAAAAAAA300.16719612104999163No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA300.16719612104999163No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA290.16162291701499193No Hit
GTGCAACAGAGCATACGTACTCTGCGTTGATAC290.16162291701499193No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA290.16162291701499193No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA280.1560497129799922No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA280.1560497129799922No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAT270.15047650894499248No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA270.15047650894499248No Hit
GCATACGTAAAAAAAAAAAAAAAAAAAAAAAAA260.14490330490999276No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC260.14490330490999276No Hit
CCCCCGCAACAGAGCATACGTACTCTGCGTTGA250.13933010087499303No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA240.1337568968399933No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA240.1337568968399933No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA240.1337568968399933No Hit
GAGTGAATGGGCAACAGAGCATAAAAAAAAAAA240.1337568968399933No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAA230.1281836928049936No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA230.1281836928049936No Hit
CAACTCAGAGTGAATGGGCAACAGAGCAAAAAA220.12261048876999388No Hit
CAACAGAGCATACGTACAAAAAAAAAAAAAAAA210.11703728473499415No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA210.11703728473499415No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA210.11703728473499415No Hit
GGGCAACAGAGCATACGTACTCTGCGTTGATAC210.11703728473499415No Hit
CCCTGCAACAGAGCATACGTACTCTGCGTTGAT210.11703728473499415No Hit
GCCCGCAACAGAGCATACGTACTCTGCGTTGAT210.11703728473499415No Hit
GCATACGTACAAAAAAAAAAAAAAAAAAAAAAA210.11703728473499415No Hit
GAGCATACAAAAAAAAAAAAAAAAAAAAAAAAA200.11146408069999444No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA200.11146408069999444No Hit
AACGCAGAGTGAATGGGCAACAGAGCATAAAAA200.11146408069999444No Hit
CATACGTACTCAAAAAAAAAAAAAAAAAAAAAA200.11146408069999444No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA190.1058908766649947No Hit
GAGTGAATGGGCAACAGAGCATACGTACTCAAA190.1058908766649947No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT190.1058908766649947No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA190.1058908766649947No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAA190.1058908766649947No Hit
CAACAGAGCATACGTACTCTGCGTTTATACCAC190.1058908766649947No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC401.7192242E-523.63480427
TATCAAC1250.018.367621
ATCAACG1401.8189894E-1216.399662
GATACCA500.00212736916.20672226
TGATACC500.00212736916.20672225
GTATCAA603.8757475E-415.7565361
TCAACGC1454.0017767E-1114.9027343
CAACGCA1703.274181E-1113.5056034
TTGATAC1001.0248701E-412.15504327
TACTCAA1358.585648E-712.0049810
GTACTCA1358.886127E-711.9714469
TCTGCGT4400.011.97087527
AACGCAG1953.3651304E-1011.7741145
GTTGATA1051.6158863E-411.5762326
CTCAAAA1401.3327408E-611.5762312
ACTCAAA1553.5708945E-711.32727911
GGGGCAA850.00547151711.0911927
CTCTGCG5050.010.43006926
TCAAAAA1606.62586E-610.12920213
ACTCTGC5400.010.0041525
Produced by FastQC (version 0.11.2)