FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate8_1.35200000060552.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate8_1.35200000060552.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42670
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA11612.720881181157722No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA9112.1349894539489105No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA5071.1881884227794703No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA4361.0217951722521676No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA3970.9303960628075931No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA3650.8554019217248653No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA2790.6538551675650339No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2780.6515116006561987No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA2560.5999531286618234No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA2560.5999531286618234No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA2460.5765174595734709No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA2260.5296461213967658No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA2140.5015233184907428No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA2090.4898054839465667No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA2050.4804312163112257No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA2050.4804312163112257No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA1970.4616826810405437No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1970.4616826810405437No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA1940.454651980314038No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1800.4218420435903445No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT1770.4148113428638388No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT1720.4030935083196625No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA1610.37731427232247483No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA1550.36325287086946334No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA1530.35856573705179284No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1490.34919146941645185No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA1460.3421607686899461No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA1400.3280993672369346No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA1380.3234122334192641No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA1340.3140379657839231No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT1320.3093508319662526No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA1290.30232013123974694No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA1260.2952894305132412No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1220.2859151628779002No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA1110.2601359268807124No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC1090.2554487930630419No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA1050.24607452542770095No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.2343566908835247No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA1000.2343566908835247No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA960.22498242324818374No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA930.217951722521678No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA900.21092102179517225No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA840.19685962034216076No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA810.189828919615655No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC810.189828919615655No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA800.1874853527068198No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA750.17576751816264355No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.1734239512538083No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.16639325052730256No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA680.1593625498007968No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC670.15701898289196156No Hit
CACACGTACTCTGAAAAAAAAAAAAAAAAAAAA670.15701898289196156No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA660.1546754159831263No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA660.1546754159831263No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA650.1523318490742911No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA630.1476447152566206No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA620.14530114834778535No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA590.13827044762127957No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA570.13358331380360908No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG570.13358331380360908No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA570.13358331380360908No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA560.13123974689477383No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA550.12889617998593858No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA540.12655261307710333No Hit
CCCATGAGCAGACACACGTACTCTGCGTTGATA490.1148347785329271No Hit
CACGTACTCTGAAAAAAAAAAAAAAAAAAAAAA480.11249121162409187No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA480.11249121162409187No Hit
GAGTAAATGGGCAGACACAAAAAAAAAAAAAAA450.10546051089758612No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA440.10311694398875088No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA430.10077337707991563No Hit
ACGCTGAGTGAATGGGCAGACACACGTACTCAA430.10077337707991563No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA800.025.31251
TATCTAC751.8189894E-1225.21
GTATCAT551.3662429E-824.5454541
GGTATCA451.6346894E-624.01
ATCTACG801.8189894E-1223.6252
TCTACGC801.8189894E-1223.6253
GAGTAAA401.7999972E-523.6251
AGTAAAT401.7999972E-523.6252
CTACGCA801.8189894E-1223.6254
TACGCAG752.7284841E-1123.4000025
CTGAGTG603.4409823E-822.54
TGAGTGA603.4409823E-822.55
ACGCTGA553.7428072E-722.090911
TAAATGG454.470683E-521.04
TATCATC658.027018E-820.769232
CGCTGAG608.6431464E-720.252
GCTGAGT608.6431464E-720.253
AACTCAG1003.6379788E-1220.2499982
GTAAATG559.31592E-619.6363643
ACTCAGA1055.456968E-1219.2857133
Produced by FastQC (version 0.11.2)