FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_92.352000000604bf.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_92.352000000604bf.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48369
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA1750.36180198060741386No Hit
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA1740.35973454071822863No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1500.3101159833777833No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA1330.27496950526163455No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC1140.2356881473671153No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA1090.22535094792118918No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.19433934958341087No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA810.167462631024003No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA780.1612603113564473No Hit
ACGCAGAGTGAATGGGCTAAACGTACTCAAAAA760.15712543157807687No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA750.15505799168889164No Hit
AACGCAGAGTGAATGGGCAGAGTGGCAAAAAAA730.1509231119105212No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA680.1405859124645951No Hit
GAGTGAATGGGCAGAGTGGCAAAAAAAAAAAAA680.1405859124645951No Hit
GTGAATGGGCTAAACGTACTCAAAAAAAAAAAA670.1385184725754099No Hit
GGGCAGAGTGGCAAAAAAAAAAAAAAAAAAAAA660.13645103268622463No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC660.13645103268622463No Hit
GCTAAACGTACTCAAAAAAAAAAAAAAAAAAAA640.1323161529078542No Hit
GAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA630.13024871301866897No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA540.11164175401600197No Hit
AACGCAGAGTGAATGGGCTAAACGTACTCAAAA530.10957431412681677No Hit
GAATGGGCAGAGTGGCAAAAAAAAAAAAAAAAA520.10750687423763154No Hit
GGACAGAGTGGCAAAAAAAAAAAAAAAAAAAAA510.10543943434844633No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA500.1033719944592611No Hit
GTGAATGGGGCAGAGTGGCAAAAAAAAAAAAAA490.10130455457007588No Hit
GTATCAACGCAGAGTGAATGGGCTAAACGTACT490.10130455457007588No Hit
CCCCCAGAGTGGCTAAACGTACTCTGCGTTGAT490.10130455457007588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG307.019531E-527.02
GTATCAT356.4121577E-627.01
TCTACGC356.4121577E-627.03
TACGCAG356.4121577E-627.05
TATCTAC307.019531E-527.01
CTACGCA356.4121577E-627.04
TATCATC401.8063476E-523.6252
ATCATCG454.486303E-521.03
TCATCGC454.486303E-521.04
CAACTCA658.082134E-820.769231
GTAGAGT404.9320806E-420.254
AACGTAG404.9320806E-420.251
ACGTAGA404.9320806E-420.252
CGTAGAG404.9320806E-420.253
AACTCAG701.7661478E-719.2857152
CGTTGAT1450.018.62069127
TATCAAC2550.018.01
ATCGCAG552.093345E-417.1818186
TAGAGTG500.002205865516.25
CATCGCA500.002205865516.25
Produced by FastQC (version 0.11.2)