FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_91.35200000060496.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_91.35200000060496.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28926
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATCCAGCTAGACGTACTCTGCGTTGATACCAC3371.1650418308787942No Hit
GTGAATGGGACATCCAGCAAAAAAAAAAAAAAA2510.8677314526723363No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1790.6188204383599529No Hit
GTGAATGGGACATCCAGCTAGAAAAAAAAAAAA1530.5289359054138145No Hit
GTGAATGGGACATCCAGAAAAAAAAAAAAAAAA1410.4874507363617507No Hit
ACATCCAGCTAGACGTACTCTGCGTTGATACCA1360.47016524925672404No Hit
ACGCAGAGTGAATGGGACATCCAGCAAAAAAAA1080.37336652146857496No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCAAA1060.36645232662656435No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1020.352623936942543No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA950.32842425499550576No Hit
GTGAATGGGACATCCAAAAAAAAAAAAAAAAAA880.3042245730484685No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA860.29731037820645784No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA840.2903961833644472No Hit
TATCAACGCAGAGTGAATGGGACATCCAGCTAG780.2696535988384153No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA740.25582520915439394No Hit
GTGAATGGGACATCCAGCTAAAAAAAAAAAAAA720.24891101431238333No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA650.22471133236534607No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT650.22471133236534607No Hit
ATCCAGCTAGACGTACTCTGCGTTGATACCACT630.21779713752333543No Hit
GAGTGAATGGGACATCCAGCAAAAAAAAAAAAA630.21779713752333543No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.19359745557629815No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA550.19014035815529282No Hit
GTGAATGGGGACATCCAAAAAAAAAAAAAAAAA540.18668326073428748No Hit
CCCACATCCAGCTAGACGTACTCTGCGTTGATA530.18322616331328218No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCTA490.16939777362926087No Hit
ACGCAGAGTGAATGGGACATCCAGCTAGAAAAA440.15211228652423425No Hit
AACGCAGAGTGAATGGGACATCCAGCAAAAAAA440.15211228652423425No Hit
CATCCAGCTAGAAAAAAAAAAAAAAAAAAAAAA430.14865518910322892No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA410.14174099426121828No Hit
GTATCAACGCAGAGTGAATGGGACATCCAGCAA400.13828389684021294No Hit
ACGCAGAGTGAATGGGACATCCAGAAAAAAAAA400.13828389684021294No Hit
AACGCAGAGTGAATGGGACATCCAGCTAGAAAA390.13482679941920764No Hit
GTGAATGGGGACATCCAGAAAAAAAAAAAAAAA360.12445550715619166No Hit
TATCAACGCAGAGTGAATGGGACATCCAGAAAA360.12445550715619166No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT340.11754131231418101No Hit
CATCCAGCTAGACGTACTCTGCGTTTATACCAC340.11754131231418101No Hit
AGCTAGACGTACTCAAAAAAAAAAAAAAAAAAA340.11754131231418101No Hit
CCCCCACATCCAGCTAGACGTACTCTGCGTTGA330.11408421489317569No Hit
CCTACATCCAGCTAGACGTACTCTGCGTTGATA320.11062711747217037No Hit
CCCCACATCCAGCTAGACGTACTCTGCGTTGAT300.10371292263015972No Hit
GAATGGGACATCCAGCAAAAAAAAAAAAAAAAA300.10371292263015972No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAATACC200.00840575127.0450611
TTCGTTG200.00847729426.9982725
CTCTTCG200.00847729426.9982722
ACTCTTC200.00847729426.9982721
TCGTTGA200.00847729426.9982726
TACTCTT200.00847729426.9982720
TCTTCGT200.00847729426.9982723
TATCAAC1850.017.5427441
ATCAACG2300.014.0860542
TCAACGC2450.013.2236423
CAACGCA2450.013.2236424
TCTGCGT7400.013.134294527
AACGCAG2900.011.1716985
GTTGATA1351.2379091E-510.99929427
TGACATC1000.001480788310.7993087
ACGCAGA5050.010.7109161
GCAGAGT4950.010.6356823
CTCTGCG8950.010.557981526
CAGAGTG5000.010.52932554
AGAGTGA5000.010.52932555
Produced by FastQC (version 0.11.2)