FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_90.3520000006046f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_90.3520000006046f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences57971
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA3450.5951251487812872No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA3410.5882251470562868No Hit
TATCAACGCAGAGTGAATGGGAACGTACTCTGC2160.3726000931500233No Hit
ACTCTGCGTTGATACAAAAAAAAAAAAAAAAAA1700.2932500733125183No Hit
TATCAACGCAGAGTGAATGGGAAACGTACTCTG1690.2915250728812682No Hit
GTATCAACGCAGAGTGAATGGGAACGTACTCTG1660.2863500715875179No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA1650.2846250711562678No Hit
GTGAATGGGAACGTACTCTGCGTTGATACAAAA1610.27772506943126735No Hit
GCGTTGATACAAAAAAAAAAAAAAAAAAAAAAA1590.27427506856876716No Hit
GAAACGTACTCTGCGTAAAAAAAAAAAAAAAAA1500.2587500646875162No Hit
ACGCAGAGTGAATGGGAACGTACTCTGCGTTGA1180.20355005088751274No Hit
GCGTTGATACCAAAAAAAAAAAAAAAAAAAAAA1070.18457504614376152No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA940.16215004053751014No Hit
GTATCAACGCAGAGTGAATGGGAAACGTACTCT910.1569750392437598No Hit
GGAACGTACTCTGCGTTGATACAAAAAAAAAAA870.1500750375187594No Hit
AACGTACTCTGCGTTAAAAAAAAAAAAAAAAAA850.14662503665625914No Hit
GTACTCTGCGTTGATACAAAAAAAAAAAAAAAA850.14662503665625914No Hit
GTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.14317503579375895No Hit
GAGTACGTTTCCAAAAAAAAAAAAAAAAAAAAA800.1380000345000086No Hit
GAAACGTACTCTGCGAAAAAAAAAAAAAAAAAA770.1328250332062583No Hit
ACTCTGCGTTGATACCAAAAAAAAAAAAAAAAA770.1328250332062583No Hit
GTGAATGGGCAGTCTCTGGAAAAAAAAAAAAAA770.1328250332062583No Hit
AACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA770.1328250332062583No Hit
AACGTACTCTGCGTTGAAAAAAAAAAAAAAAAA760.1311000327750082No Hit
GAAACGTACTCTGCGTTGATACAAAAAAAAAAA740.12765003191250798No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA740.12765003191250798No Hit
GTCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA740.12765003191250798No Hit
GTGAATGGGAACGTACTCTGCGTTGATACCAAA730.1259250314812579No Hit
AACGCAGAGTGAATGGGAACGTACTCTGCGTTG710.12247503061875765No Hit
GAACGTACTCTGCGTTGATACAAAAAAAAAAAA700.12075003018750755No Hit
GTATCATCGCAGAGTGAATGGGAACGTACTCTG690.11902502975625744No Hit
GGTATCAACGCAGAGTGAATGGGAACGTACTCT670.11557502889375723No Hit
AACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA670.11557502889375723No Hit
ACGCAGAGTGAATGGGAAACGTACTCTGCGTAA650.11212502803125701No Hit
GTGAATGGGAAACGTACTCTGCGTAAAAAAAAA650.11212502803125701No Hit
TATCAACGCAGAGTGAATGGGCAGTCTCTGGAA630.1086750271687568No Hit
GAGTGAATGGGAACGTACTCTGCGTTGATACAA630.1086750271687568No Hit
GTCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA590.10177502544375637No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG451.6517188E-624.02
TCTACGC451.6517188E-624.03
GTATCAT401.8145498E-523.6251
TATCTAC401.8145498E-523.6251
TATCATC401.8145498E-523.6252
CTACGCA401.8145498E-523.6254
TACGCAG504.125077E-621.5999985
TCATCGC454.5064764E-521.04
CATCGCA454.5064764E-521.05
TCGCAGA501.0133295E-418.97
ATCATCG501.0133295E-418.93
ATCGCAG552.1025754E-417.1818186
CTCCCAG500.00221311716.1999995
AACCAGT500.00221311716.1999996
TGCGTAA1104.663889E-915.95454410
AACAGTC1104.663889E-915.9545447
CGCCAGT604.082491E-415.756
GTATCAA2500.015.121
ATCCAGT550.00417673714.7272726
GCACAGT550.00417673714.7272726
Produced by FastQC (version 0.11.2)