FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_85.352000000603cd.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_85.352000000603cd.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30187
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA5941.9677344552290719No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA2680.8877993838407261No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA2680.8877993838407261No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA2200.7287905389737304No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2080.6890383277569815No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA1670.5532182727664227No Hit
CGGTACTAGAGACGTACTCTGCGTTGATACCAC1480.49027727167323687No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA1320.4372743233842382No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA1270.4207109020439262No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA1170.3875840593633021No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA990.32795574253817866No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.31139232119786664No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA770.2550766886408057No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA770.2550766886408057No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA590.19544837181568225No Hit
CTCTAGTACCAAAAAAAAAAAAAAAAAAAAAAA510.16894689767118296No Hit
GTGAATGGGTACTAGAGACGTACTCAAAAAAAA480.15900884486699574No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT480.15900884486699574No Hit
GTGAATGGGGTCGGTACAAAAAAAAAAAAAAAA440.1457581077947461No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.1457581077947461No Hit
CGGTACTAGAGAAAAAAAAAAAAAAAAAAAAAA440.1457581077947461No Hit
ACGCAGAGTGAATGGGTCGGTACAAAAAAAAAA410.13582005499055885No Hit
GGTCGGTACAAAAAAAAAAAAAAAAAAAAAAAA390.12919468645443402No Hit
TATCAACGCAGAGTGAATGGGTCGGTACTAGAG390.12919468645443402No Hit
CTCTAGTACCGACAAAAAAAAAAAAAAAAAAAA370.12256931791830922No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC370.12256931791830922No Hit
TATCAACGCAGAGTGAATGGGATCGGTACTAGA350.11594394938218439No Hit
GTGAATGGGATCGGTACAAAAAAAAAAAAAAAA350.11594394938218439No Hit
GAGTGAATGGGATCGGTACAAAAAAAAAAAAAA350.11594394938218439No Hit
GTGAATGGGGGAGTCGGAAAAAAAAAAAAAAAA340.11263126511412197No Hit
GTACTAGAGACGTACTAAAAAAAAAAAAAAAAA330.10931858084605955No Hit
GTGAATGGGTCGGTACTAAAAAAAAAAAAAAAA320.10600589657799714No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA320.10600589657799714No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAACTCA257.6511287E-427.01
GTATCAT405.7563375E-727.01
CGTACAA306.93343E-527.011
TATCATC405.7563375E-727.02
ATCTACG451.6080267E-624.02
TCTACGC451.6080267E-624.03
TACGCAG451.6080267E-624.05
TATCTAC451.6080267E-624.01
CTACGCA451.6080267E-624.04
ACGTACA401.777149E-523.62510
TGGGAGG300.002214162122.56
TACCGAG404.8729067E-420.257
TCGCAGA559.166546E-619.6363647
ATCATCG559.166546E-619.6363643
ATCGCAG559.166546E-619.6363646
TCATCGC559.166546E-619.6363644
CATCGCA559.166546E-619.6363645
TCAGAGT350.00541121619.2857155
TATCAAC2250.019.1999991
TCTGCGT4650.018.29032127
Produced by FastQC (version 0.11.2)