FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_84.352000000603a4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_84.352000000603a4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30306
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTCGTTCTGACGTACTCTGCGTTGATACCAC2250.7424272421302712No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1620.5345476143337953No Hit
GTGAATGGGACTGTCGTAAAAAAAAAAAAAAAA1620.5345476143337953No Hit
GTGAATGGGACTGTCGAAAAAAAAAAAAAAAAA1410.4652544050683033No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT910.3002705734837986No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA810.26727380716689764No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA800.2639741305352075No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA680.2243780109549264No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA650.2144789810598561No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA600.19798059790140568No Hit
CTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1847818913746453No Hit
GTGAATGGGGACTGTCGAAAAAAAAAAAAAAAA510.1682835082161948No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA490.16168415495281463No Hit
ACGCAGAGTGAATGGGACTGTCGTAAAAAAAAA490.16168415495281463No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA490.16168415495281463No Hit
ACGCAGAGTGAATGGGACTGTCGAAAAAAAAAA490.16168415495281463No Hit
GTCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.15838447832112454No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA470.15508480168943442No Hit
CCACTGTCGTTCTGACGTACTCTGCGTTGATAC460.15178512505774433No Hit
GAGTGAATGGGACTGTCGAAAAAAAAAAAAAAA460.15178512505774433No Hit
TATCAACGCAGAGTGAATGGGACTGTCGAAAAA440.14518577179436415No Hit
GAGTGAATGGGACTGTCGTAAAAAAAAAAAAAA420.13858641853098397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA400.13198706526760376No Hit
GAATGGGACTGTCGTAAAAAAAAAAAAAAAAAA400.13198706526760376No Hit
GAATGGGACTGTCGAAAAAAAAAAAAAAAAAAA390.12868738863591367No Hit
TATCAACGCAGAGTGAATGGGACTGTCGTAAAA380.1253877120042236No Hit
GTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.1220880353725335No Hit
GTTCTGACGTACTCAAAAAAAAAAAAAAAAAAA350.1154886821091533No Hit
GTGAATGGGGACTGTCGTAAAAAAAAAAAAAAA330.10888932884577311No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT257.652022E-427.01
TATCATC257.652022E-427.02
ATCTACG351.9759545E-423.1428592
TCTACGC351.9759545E-423.1428593
TACGCAG351.9759545E-423.1428595
TATCTAC351.9759545E-423.1428591
CTACGCA351.9759545E-423.1428594
CGTCACT300.002214416622.55
TCGCAGA300.002214416622.57
ATCATCG300.002214416622.53
ATCGCAG300.002214416622.56
TCATCGC300.002214416622.54
CATCGCA300.002214416622.55
GTCACTG404.873685E-420.256
TCTGCGT9100.018.8406627
TCACTGT1207.9853635E-1015.757
TATCAAC2100.015.4285711
CTCTGCG11300.015.05309726
GAGTACG550.00411554614.7272731
ACTCTGC12400.013.82661225
Produced by FastQC (version 0.11.2)