FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_82.35200000060360.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_82.35200000060360.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14163
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA2021.4262515003883358No Hit
GAGTGAATGGGATCGAGACAAAAAAAAAAAAAA1270.896702675986726No Hit
GTGAATGGGATCGAGACAAAAAAAAAAAAAAAA1060.7484290051542752No Hit
GATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA820.57897338134576No Hit
ACGCAGAGTGAATGGGATCGAGACAAAAAAAAA810.5719127303537386No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA740.5224881734095884No Hit
GTGAATGGGACGAGTACAAAAAAAAAAAAAAAA570.4024571065452235No Hit
TATCAACGCAGAGTGAATGGGATCGAGACAAAA560.395396455553202No Hit
AACGCAGAGTGAATGGGATCGAGACAAAAAAAA480.3389112476170303No Hit
ATCGAGACGAGAAAAAAAAAAAAAAAAAAAAAA480.3389112476170303No Hit
ACGCAGAGTGAATGGGATCGAGACGAGAAAAAA470.3318505966250088No Hit
GTGAATGGGATCGAGACGAGAAAAAAAAAAAAA460.32478994563298735No Hit
GAGTGAATGGGATCGAGACGAGAAAAAAAAAAA450.3177292946409659No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA440.3106686436489444No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC400.2824260396808586No Hit
GAGTGAATGGGACGAGTACAAAAAAAAAAAAAA390.2753653886888371No Hit
GACGAGTACAAAAAAAAAAAAAAAAAAAAAAAA370.2612440867047942No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGA360.25418343571277274No Hit
GAGTGAATGGGATCGAGACGAAAAAAAAAAAAA350.24712278472075125No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC350.24712278472075125No Hit
ACGCAGAGTGAATGGGACGAGTACAAAAAAAAA350.24712278472075125No Hit
TATCAACGCAGAGTGAATGGGACGAGTACAAAA340.24006213372872978No Hit
ACGCAGAGTGAATGGGATCGAGACGAAAAAAAA330.23300148273670834No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA330.23300148273670834No Hit
GACGAGTACGTACTCAAAAAAAAAAAAAAAAAA320.22594083174468688No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA310.21888018075266538No Hit
AACGCAGAGTGAATGGGACGAGTACAAAAAAAA300.21181952976064394No Hit
AGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA290.20475887876862248No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT290.20475887876862248No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA270.19063757678457952No Hit
TATCAACGCAGAGTGAATGGGACGAGTACGTAC270.19063757678457952No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA270.19063757678457952No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACAAA250.1765162748005366No Hit
AACGCAGAGTGAATGGGATCGAGACGAGAAAAA250.1765162748005366No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA250.1765162748005366No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAGT250.1765162748005366No Hit
ATCAACGCAGAGTGAATGGGATCGAGACAAAAA240.16945562380851514No Hit
GATCGAGACGAGAAAAAAAAAAAAAAAAAAAAA240.16945562380851514No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA230.16239497281649368No Hit
GTGAATGGGATCGAGACGAAAAAAAAAAAAAAA230.16239497281649368No Hit
GTATCAACGCAGAGTGAATGGGATCGAGACGAG220.1553343218244722No Hit
GAATGGGATCGAGACAAAAAAAAAAAAAAAAAA220.1553343218244722No Hit
GAATGGGACGAGTACAAAAAAAAAAAAAAAAAA210.14827367083245074No Hit
AGACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.14827367083245074No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA200.1412130198404293No Hit
CCATCGAGACGAGTACGTACTCTGCGTTGATAC200.1412130198404293No Hit
GTGAATGGGGATCGAGACAAAAAAAAAAAAAAA190.1341523688484078No Hit
GTGAATGGGGATCGAGAAAAAAAAAAAAAAAAA190.1341523688484078No Hit
AGTACGTACAAAAAAAAAAAAAAAAAAAAAAAA190.1341523688484078No Hit
GTGAATGGGACGAGAAAAAAAAAAAAAAAAAAA180.12709171785638637No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT180.12709171785638637No Hit
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA180.12709171785638637No Hit
TATCAACGCAGAGTGAATGGGATCGAGACGAAA180.12709171785638637No Hit
GTGAATGGGACGAGTAAAAAAAAAAAAAAAAAA180.12709171785638637No Hit
AACGCAGAGTGAATGGGATCGAGACGAAAAAAA170.12003106686436489No Hit
TATCGAGACAAAAAAAAAAAAAAAAAAAAAAAA170.12003106686436489No Hit
ACGCAGAGTGAATGGGACGAGTAAAAAAAAAAA170.12003106686436489No Hit
GTGAATGGGAATCGAGACGAGAAAAAAAAAAAA170.12003106686436489No Hit
ATCGAGACGAGTACAAAAAAAAAAAAAAAAAAA170.12003106686436489No Hit
GAGTGAATGGGACGAGTAAAAAAAAAAAAAAAA160.11297041587234344No Hit
GTATCGAGACGAGTACGTACTCTGCGTTGATAC160.11297041587234344No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA150.10590976488032197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCAAC1450.016.758621
ATCAACG1503.6379788E-1215.2999992
TCAACGC1557.2759576E-1214.8064533
CAACGCA1808.0035534E-1112.754
GCGTTGA1101.7300938E-512.27272727
TCTGCGT3000.011.24999927
GTATCAA850.005279939611.1176461
CTCTGCG3250.010.38461626
TGGGATC1503.584873E-59.913
AACGCAG2409.566065E-99.56255
ACTCTGC3450.09.39130525
TGCGTTG1452.8767824E-49.31034426
TACTCTG3500.09.25714324
ATGGGAT1651.0201169E-49.012
GTACTCT3651.8189894E-128.87671323
GGGATCG1701.4127142E-48.73529314
AATGGGG1500.00417115038.0999994
GTGAATG5950.07.9411771
CTGCGTT1700.00140676177.94117625
CGTACTC4102.910383E-117.902438622
Produced by FastQC (version 0.11.2)