FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_80.3520000006032d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_80.3520000006032d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55389
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC7621.3757244218166063No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA7171.2944808535990902No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA6751.2186535232627418No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA5510.9947823575078084No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA3840.6932784487894708No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA3420.6174511184531224No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA3410.6156457058260665No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA3390.6120348805719547No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA3280.5921753416743397No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA3180.5741212154037805No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA3060.5524562638791096No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA2830.5109317734568236No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA2700.4874614093050966No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA2640.4766289335427612No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA2290.41343949159580423No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA2180.39357995269818913No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT2060.37191500117351817No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA1900.3430283991406236No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1870.33761216125945587No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC1830.33039051075123216No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA1550.2798389571936666No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA1520.2744227193124989No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA1470.2653956561772193No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA1420.2563685930419397No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA1420.2563685930419397No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA1360.24553611727960423No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA1340.2419252920254924No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA1220.22026034050082144No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1210.21845492787376553No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA1210.21845492787376553No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC1180.2130386899925978No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC1180.2130386899925978No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA1170.2112332773655419No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA1170.2112332773655419No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC1160.20942786473848599No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.20581703948437416No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA1130.20401162685731827No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC1120.2022062142302623No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA1110.2004008016032064No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA1030.18595750058675908No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA990.17873585007853546No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA990.17873585007853546No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA980.17693043745147954No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG930.16790337431619998No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA920.16609796168914406No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA910.16429254906208812No Hit
GTGAATGGGCTAACTATAAAAAAAAAAAAAAAA890.16068172380797632No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACGTA850.15346007329975264No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA850.15346007329975264No Hit
GTGAATGGGGCTAACTATCCAAAAAAAAAAAAA850.15346007329975264No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA830.14984924804564084No Hit
GGCTAACTATCCACGTACAAAAAAAAAAAAAAA820.14804383541858493No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA820.14804383541858493No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA810.14623842279152902No Hit
TATCTACGCAGAGTGAATGGGCTAACTATCCAC790.14262759753741716No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA760.13721135965624945No Hit
ATCAACGCAGAGTGAATGGGGCTAACTATCCAC760.13721135965624945No Hit
GAATGGGGCTAACTATCCACAAAAAAAAAAAAA730.13179512177508168No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA710.12818429652096985No Hit
ACGCAGAGTGAATGGGCTAACTAAAAAAAAAAA700.12637888389391397No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA690.12457347126685805No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA660.1191572333856903No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA650.11735182075863439No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA640.11554640813157847No Hit
TATCAACGCAGAGTGAATGGGCTAACTATAAAA640.11554640813157847No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA630.11374099550452256No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA630.11374099550452256No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC610.11013017025041072No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA610.11013017025041072No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA590.1065193449962989No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA590.1065193449962989No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA590.1065193449962989No Hit
GTGAATGGGGCTAACTATAAAAAAAAAAAAAAA580.10471393236924299No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA560.10110310711513115No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG307.020266E-527.0099562
TCTACGC307.020266E-527.0099563
TATCTAC307.020266E-527.0099561
GTATCAT554.620233E-1027.0099541
TATCATC554.620233E-1027.0099542
TACGCAG307.063743E-526.9855355
CTACGCA307.063743E-526.9855354
ATCATCG707.550625E-921.2221073
ATCGCAG707.6306605E-921.2029196
TCATCGC707.6306605E-921.2029194
CATCGCA707.6306605E-921.2029195
CAACTCA454.488891E-521.0077421
CAGGGCT404.962686E-420.2391516
TCGCAGA751.688386E-819.7893927
AACTCAG501.00939775E-418.906972
TATCAAC4850.018.656361
CACGGCT450.001096101417.9903556
TGCGTTG4400.017.49508527
ACTCAGA552.0944636E-417.1881523
CTCAGAG552.1090358E-417.1726114
Produced by FastQC (version 0.11.2)