FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_79.35200000060303.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_79.35200000060303.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47289
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGACATCGCAAAAAAAAAAAAAAAAA2360.4990589777749583No Hit
GTGAATGGGGACATCGCAAAAAAAAAAAAAAAA2080.43984859058131914No Hit
GTGAATGGGATGTCTGACGTACTCAAAAAAAAA2070.437733933895832No Hit
ACGTCAGACATCGCAAAAAAAAAAAAAAAAAAA1980.41870202372644805No Hit
GAGTACGTCAGACATCGCAAAAAAAAAAAAAAA1570.33200109962147645No Hit
TGTCTGACGTACTCAAAAAAAAAAAAAAAAAAA1500.3171985028230667No Hit
TATCAACGCAGAGTGAATGGGATGTCTGACGTA1500.3171985028230667No Hit
GTCTGACGTACTCAAAAAAAAAAAAAAAAAAAA1140.24107086214553067No Hit
ACGCAGAGTGAATGGGGACATCGCAAAAAAAAA1140.24107086214553067No Hit
GAGTGAATGGGATGTCTGACGTACTCAAAAAAA1120.23684154877455643No Hit
ACGCAGAGTGAATGGGATGTCTGACGTACTCAA960.20300704180676266No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA950.20089238512127558No Hit
GGGTCAGACATCGCAAAAAAAAAAAAAAAAAAA930.19666307175030132No Hit
GACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA900.19031910169384No Hit
GTACGTCAGACATCGCAAAAAAAAAAAAAAAAA880.18608978832286577No Hit
CTGACGTACTCAAAAAAAAAAAAAAAAAAAAAA860.18186047495189156No Hit
GAGTGAATGGGGACATCGCAAAAAAAAAAAAAA780.16494322146799467No Hit
GAATGGGGACATCGCAAAAAAAAAAAAAAAAAA770.16282856478250757No Hit
GAATGGGACATCGCAAAAAAAAAAAAAAAAAAA750.15859925141153336No Hit
ACGCAGAGTGAATGGGACATCGCAAAAAAAAAA730.1543699380405591No Hit
ATGTCTGACGTACTCAAAAAAAAAAAAAAAAAA670.14168199792763647No Hit
GAGTACGTCAGACAAAAAAAAAAAAAAAAAAAA670.14168199792763647No Hit
GTATCAACGCAGAGTGAATGGGATGTCTGACGT650.1374526845566622No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA630.133223371185688No Hit
GTCAGACATCGCAAAAAAAAAAAAAAAAAAAAA600.1268794011292267No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA590.12476474444373957No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA590.12476474444373957No Hit
GAGTACGTCAAAAAAAAAAAAAAAAAAAAAAAA580.12265008775825244No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT570.12053543107276533No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA570.12053543107276533No Hit
GGACATCGCAAAAAAAAAAAAAAAAAAAAAAAA560.11842077438727822No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA530.1120768043308169No Hit
GTACGTAGACATCGCAAAAAAAAAAAAAAAAAA500.10573283427435555No Hit
GACATCGCAAAAAAAAAAAAAAAAAAAAAAAAA500.10573283427435555No Hit
TATCAACGCAGAGTGAATGGGGACATCGCAAAA480.10150352090338133No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGAGTG307.054195E-526.9788364
ACGCTGA307.054195E-526.9788361
GTATCAT455.4442353E-826.9788361
TGAGTGA307.054195E-526.9788365
TATCATC451.6522044E-623.9811882
CGCTGAG352.0097317E-423.1247162
GCTGAGT352.0097317E-423.1247163
ATTTGCG300.002243068522.4823638
TCGCAGA559.41464E-619.6209727
ATCGCAG559.41464E-619.6209726
ATCATCG601.9934334E-517.985893
TCATCGC601.9934334E-517.985894
TGCGTTG2600.014.03795227
TGGGACA1252.5080226E-814.02899556
GGATTGC802.3275573E-413.4894188
GGGATTG600.00744973813.4894187
TGGGATT700.001314435913.4894176
TATCAAC3450.013.293921
TCTGCGT6050.012.73599327
ATCAACG3900.012.1058872
Produced by FastQC (version 0.11.2)