FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_76.35200000060298.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_76.35200000060298.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30547
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCGTCCTCTACGTACTCTGCGTTGATACCA4311.410940517890464No Hit
CTCGTCCTCTACGTACTCTGCGTTGATACCACT2240.7332962320358791No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1590.5205093789897535No Hit
GTGAATGGGATCTCGTCCAAAAAAAAAAAAAAA1490.4877729400595803No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1210.39611091105509544No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1200.3928372671620781No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1120.36664811601793956No Hit
GAGTGAATGGGATCTCGTCCAAAAAAAAAAAAA1100.3601008282319049No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1050.3437326087668184No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA980.32081710151569715No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA980.32081710151569715No Hit
GTGAATGGGATCTCGTCAAAAAAAAAAAAAAAA940.30772252594362787No Hit
ACGCAGAGTGAATGGGATCTCGTCCAAAAAAAA930.30444888205061055No Hit
GTGAATGGGATCTCGTCCTCAAAAAAAAAAAAA880.28808066258552395No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCAAA870.28480701869250663No Hit
CATCTCGTCCTCTACGTACTCTGCGTTGATACC840.27498608701345467No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA800.2618915114413854No Hit
GGTACGTAGAGGACGAAAAAAAAAAAAAAAAAA630.20623956526009102No Hit
AACGCAGAGTGAATGGGATCTCGTCCAAAAAAA570.1865977019019871No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA530.17350312632991785No Hit
GTAGAGGACGAAAAAAAAAAAAAAAAAAAAAAA530.17350312632991785No Hit
ACGCAGAGTGAATGGGATCTCGTCCTCAAAAAA520.1702294824369005No Hit
GTCCTCTACGTACTCAAAAAAAAAAAAAAAAAA520.1702294824369005No Hit
ACGCAGAGTGAATGGGATCTCGTCAAAAAAAAA490.16040855075784857No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCA490.16040855075784857No Hit
GTACGTAGAGGACGAAAAAAAAAAAAAAAAAAA490.16040855075784857No Hit
GAGTACGTAGAGGACGAAAAAAAAAAAAAAAAA480.15713490686483123No Hit
GAGTGAATGGGATCTCGTCCTCAAAAAAAAAAA450.1473139751857793No Hit
GTGAATGGGGTACGTAGAGGACGAAAAAAAAAA450.1473139751857793No Hit
GTGAATGGGAATCTCGTCCTCAAAAAAAAAAAA440.14404033129276198No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA430.14076668739974466No Hit
GAGTGAATGGGATCTCGTCAAAAAAAAAAAAAA420.13749304350672734No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCAAAA410.13421939961371004No Hit
GTATCAACGCAGAGTGAATGGGATCTCGTCCAA400.1309457557206927No Hit
GATCTCGTCCTCTACGTACTCTGCGTTGATACC400.1309457557206927No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.12767211182767538No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA380.12439846793465806No Hit
GTGAATGGGGATCTCGTCCAAAAAAAAAAAAAA380.12439846793465806No Hit
GTGAATGGGATCTCGTCCTAAAAAAAAAAAAAA360.11785118014862343No Hit
ACGCAGAGTGAATGGGCATCTCGTCCTCAAAAA360.11785118014862343No Hit
GATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAA350.1145775362556061No Hit
TATCAACGCAGAGTGAATGGGATCTCGTCCTCT350.1145775362556061No Hit
ATCTCGTCCTCAAAAAAAAAAAAAAAAAAAAAA350.1145775362556061No Hit
GATCTCGTCCAAAAAAAAAAAAAAAAAAAAAAA350.1145775362556061No Hit
GAATGGGATCTCGTCCAAAAAAAAAAAAAAAAA340.11130389236258881No Hit
GTGAATGGGGATCTCGTCAAAAAAAAAAAAAAA330.10803024846957147No Hit
AACGCAGAGTGAATGGGATCTCGTCCTCAAAAA310.10148296068353685No Hit
GTGAATGGGGATCTCGAAAAAAAAAAAAAAAAA310.10148296068353685No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA310.10148296068353685No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTACGC351.972196E-423.1499043
TACGCAG351.972196E-423.1499045
TATCTAC351.972196E-423.1499041
CTACGCA351.972196E-423.1499044
ATCTACG404.8644535E-420.2561682
TATCAAC2750.018.1691681
GCGTTGA2350.016.09000627
ATCAACG3200.015.1921272
TCAACGC3300.015.1409743
ATGGGGC657.335938E-414.5428917
CAACGCA3350.014.5118814
TGCGTTG4650.013.06849627
AACGCAG3950.011.9656695
CTGCGTT5800.010.71015726
TCATCTC900.008536668510.4859527
CTATCTC900.008536668510.4859527
TCTGCGT9550.010.32251527
ACGCAGA5750.09.6132086
CTCTGCG10600.09.30000126
GCATCTC1453.179087E-49.2978897
Produced by FastQC (version 0.11.2)