FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_75.3520000006027e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_75.3520000006027e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46992
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1710.3638917262512768No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT930.19790602655771197No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA820.1744977868573374No Hit
GAGTACGTTTCGACCAAAAAAAAAAAAAAAAAA760.16172965611167858No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA750.15960163432073546No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA720.15321756894790603No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA710.15108954715696288No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA700.14896152536601975No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA630.13406537282941777No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA610.1298093292475315No Hit
GTGAATGGGGTACGTTTCGACCAAAAAAAAAAA590.12555328566564522No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA590.12555328566564522No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA580.12342526387470207No Hit
GTGAATGGGGAGTACGTTTCGACCAAAAAAAAA560.1191692202928158No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA560.1191692202928158No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC510.10852911133810009No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAGAG200.00853784626.993617
ATGGGCG300.002235732522.49467517
TCTGCGT6550.015.47083427
TTTCGTT550.004167612614.7237877
TCTCGTT1057.719591E-714.139518
ATGGGGG802.3162883E-413.4968055
TATCAAC2550.013.2321621
TGCGTTG2151.8189894E-1212.56855327
GCGTTGA1203.42356E-612.38526227
CTCTGCG8700.011.64758126
ATCAACG2950.011.4379712
CATCGTT850.005612540511.1150177
TCAACGC3100.010.884523
ACTCTGC9450.010.580193525
CTGCGTT2707.2759576E-1210.50870626
TACTCTG9600.010.41487924
GTACTCT9800.010.2023323
GTATCAA2801.4551915E-1110.1226041
CGTACTC10100.09.89929122
TGGTCGA10350.09.78029313
Produced by FastQC (version 0.11.2)