FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_67.3520000006017f.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_67.3520000006017f.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences42728
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCTCTGAACAAAAAAAAAAAAAAAA4381.0250889346564314No Hit
GAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4170.9759408350496163No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAC4170.9759408350496163No Hit
ACGCAGAGTGAATGGGCTCTGAACAAAAAAAAA3430.8027522935779817No Hit
GCTCTGAACGTACTCAAAAAAAAAAAAAAAAAA2940.6880733944954129No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACAAAA2810.6576483804530987No Hit
GAGTGAATGGGCTCTGAACAAAAAAAAAAAAAA2730.6389252948885976No Hit
GTGAATGGGCTCTGAAAAAAAAAAAAAAAAAAA2620.6131810522374087No Hit
GCTCTGAACAAAAAAAAAAAAAAAAAAAAAAAA2540.5944579666729076No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGTA2480.580415652499532No Hit
ACGCAGAGTGAATGGGCTCTGAAAAAAAAAAAA2450.573394495412844No Hit
ACGCAGAGTGAATGGGCTCTGAACGAAAAAAAA2390.5593521812394683No Hit
GTGAATGGGCTCTGAACGAAAAAAAAAAAAAAA2240.5242463958060288No Hit
GTGAATGGGCTCTGAACGTACTCAAAAAAAAAA2010.47041752480808835No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGAAA2000.46807713911252574No Hit
GTGAATGGGAATGCGCTCAAAAAAAAAAAAAAA1960.45871559633027525No Hit
GAGTGAATGGGCTCTGAAAAAAAAAAAAAAAAA1900.44467328215689944No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCAAA1840.4306309679835237No Hit
GAGTGAATGGGCTCTGAACGAAAAAAAAAAAAA1820.42595019659239847No Hit
AACGCAGAGTGAATGGGCTCTGAACAAAAAAAA1790.41892903950571053No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACTCTGC1760.41190788241902265No Hit
TATCAACGCAGAGTGAATGGGCTCTGAAAAAAA1750.40956749672346004No Hit
GCTCTGAACGTACAAAAAAAAAAAAAAAAAAAA1670.39084441115895896No Hit
CTCTGAACGTACTCAAAAAAAAAAAAAAAAAAA1650.38616363976783374No Hit
GAGTGAATGGGCTCTGAACGTACTCAAAAAAAA1500.3510578543343943No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGTA1500.3510578543343943No Hit
GAACGTACTCTGCGTAAAAAAAAAAAAAAAAAA1350.3159520689009549No Hit
AACGCAGAGTGAATGGGCTCTGAACGAAAAAAA1320.30893091181426696No Hit
GTGAATGGGCTCTGAACGTACAAAAAAAAAAAA1310.30659052611870435No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACAAA1300.30425014042314175No Hit
GAACGTACTCAAAAAAAAAAAAAAAAAAAAAAA1240.290207826249766No Hit
AACGCAGAGTGAATGGGCTCTGAAAAAAAAAAA1190.27850589777195284No Hit
GCTCTGAACGAAAAAAAAAAAAAAAAAAAAAAA1160.27148474068526496No Hit
ACGCAGAGTGAATGGGCTCTGAACGTACAAAAA1050.24574049803407602No Hit
GTGAATGGGAATGCGCAAAAAAAAAAAAAAAAA990.23169818386070024No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACAAAAA970.22701741246957502No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACAAA960.22467702677401233No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAACGAA920.21531548399176184No Hit
GAGTGAATGGGCTCTGAACGTACAAAAAAAAAA880.20595394120951133No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGTACT860.20127316981838608No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCTG840.19659239842726078No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGTAC820.19191162703613554No Hit
GTATCAACGCAGAGTGAATGGGCTCTGAAAAAA800.1872308556450103No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACTCAA800.1872308556450103No Hit
GAACGTACAAAAAAAAAAAAAAAAAAAAAAAAA800.1872308556450103No Hit
GTGAATGGGCTCTGAACGTACTCTGCGTTGATA760.17786931286275978No Hit
CAACGCAGAGTGAATGGGCTCTGAACAAAAAAA750.17552892716719715No Hit
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA730.17084815577607187No Hit
GAACGAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.1614866129938214No Hit
ATCAACGCAGAGTGAATGGGCTCTGAAAAAAAA680.15914622729825875No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA680.15914622729825875No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA670.15680584160269612No Hit
ATCAACGCAGAGTGAATGGGCTCTGAACGAAAA650.15212507021157087No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACGT640.14978468451600824No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACAAAA630.1474442988204456No Hit
ACGCAGAGTGAATGGGCTCAAAAAAAAAAAAAA620.145103913124883No Hit
GTGAATGGGCTCTGAACGTAAAAAAAAAAAAAA620.145103913124883No Hit
TATCAACGCAGAGTGAATGGGAATGCGCTCAAA600.14042314173375772No Hit
AACGCAGAGTGAATGGGCTCTGAACGTACAAAA600.14042314173375772No Hit
CAACTCAGAGTGAATGGGCTCTGAACAAAAAAA600.14042314173375772No Hit
CAACGCAGAGTGAATGGGCTCTGAACGAAAAAA590.1380827560381951No Hit
AATGCGCTCTGAACGTACTCTGCGTTGATACCA580.13574237034263248No Hit
GTGAATGGGAATGCGCTAAAAAAAAAAAAAAAA570.13340198464706984No Hit
GCTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAA560.1310615989515072No Hit
ACGCAGAGTGAATGGGAATGCGCTCAAAAAAAA560.1310615989515072No Hit
AACGTAGAGTGAATGGGCTCTGAACAAAAAAAA550.1287212132559446No Hit
CTGAACGTACTCTGCGAAAAAAAAAAAAAAAAA550.1287212132559446No Hit
GAGTGAATGGGCTCAAAAAAAAAAAAAAAAAAA540.12638082756038194No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAAAAAA530.12404044186481933No Hit
TATCTACGCAGAGTGAATGGGCTCTGAACGAAA510.11935967047369406No Hit
TATCAACGCAGAGTGAATGGGCTCTGAACGTAA510.11935967047369406No Hit
TATCAACGCAGAGTGAATGGGCTCAAAAAAAAA500.11701928477813144No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.11467889908256881No Hit
CTGAACGTACTCTGCGTAAAAAAAAAAAAAAAA480.11233851338700616No Hit
GAATGGGCTCTGAACAAAAAAAAAAAAAAAAAA470.10999812769144354No Hit
GAGTGAATGGGCTCTGAACGTACTCTGCGTTGA450.10531735630031828No Hit
GTATCATCGCAGAGTGAATGGGCTCTGAACGAA450.10531735630031828No Hit
TCAACGCAGAGTGAATGGGCTCTGAACAAAAAA440.10297697060475566No Hit
CAACGCAGAGTGAATGGGCTCTGAAAAAAAAAA440.10297697060475566No Hit
GGTATCAACGCAGAGTGAATGGGCTCTGAACAA440.10297697060475566No Hit
CAACTCAGAGTGAATGGGCTCTGAACGTACTCT440.10297697060475566No Hit
GAACGTACTCTGCGTTGATACCAAAAAAAAAAA440.10297697060475566No Hit
TATCAACGCAGAGTGAATGGGCTCTTAACAAAA440.10297697060475566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAT401.8089884E-523.6095751
TATCATC401.8089884E-523.6095752
CTGAACT300.002222429722.51176520
ACGCTGA300.00223771122.485311
GGTATCA751.663102E-819.7870731
GACAATG350.00546817219.2731256
TGAGTGA350.00546817219.2731255
AACGTAG450.001091299517.9882491
TTCAATG450.001091299517.9882496
CTTAATG450.001091299517.9882493
TATCAAC3600.017.6134951
ACGTAGA500.002209750216.1894252
CGTAGAG500.002209750216.1894253
TCGCAGA604.0709047E-415.7397177
ATCATCG604.0709047E-415.7397173
ATCGCAG604.0709047E-415.7397176
CATCGCA604.0709047E-415.7397175
TCAATGC1559.094947E-1214.7967867
TCATCGC657.476236E-414.528974
ATCAACG4400.014.1044232
Produced by FastQC (version 0.11.2)