FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_64.35200000060112.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_64.35200000060112.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39009
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTATGGTGTTCACGTACTCTGCGTTGATACCA5181.327898690045887No Hit
GTGAATGGGCTATGGTGAAAAAAAAAAAAAAAA3190.8177600041016175No Hit
CCGCTATGGTGTTCACGTACTCTGCGTTGATAC2410.6178061472993412No Hit
GTGTTCACGTACTCAAAAAAAAAAAAAAAAAAA2220.5690994385910944No Hit
GTGAATGGGCTATGGTGTAAAAAAAAAAAAAAA1830.4691225101899562No Hit
ACGCAGAGTGAATGGGCTATGGTGAAAAAAAAA1670.42810633443564305No Hit
GCGCTATGGTGTTCACGTACTCTGCGTTGATAC1520.3896536696659745No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1250.3204388730805711No Hit
GTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1230.315311851111282No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGAAAA1190.30505780717270375No Hit
ACGCAGAGTGAATGGGCTATGGTGTAAAAAAAA1180.3024942961880592No Hit
ACGCTATGGTGTTCACGTACTCTGCGTTGATAC1170.2999307852034146No Hit
GTGAATGGGGCTATGGTGAAAAAAAAAAAAAAA1120.28711323028019176No Hit
GGGCTATGGTGAAAAAAAAAAAAAAAAAAAAAA1120.28711323028019176No Hit
GGTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1090.279422697326258No Hit
GGGCTATGGTGTAAAAAAAAAAAAAAAAAAAAA1070.2742956753569689No Hit
CTGCTATGGTGTTCACGTACTCTGCGTTGATAC1060.27173216437232434No Hit
CTATGGTGTTCACGTACTCTGCGTTGATACCAC1000.2563510984644569No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTAAA970.2486605655105232No Hit
AACGCAGAGTGAATGGGCTATGGTGAAAAAAAA970.2486605655105232No Hit
GAGTGAATGGGCTATGGTGAAAAAAAAAAAAAA900.23071598861801124No Hit
GTGCTATGGTGTTCACGTACTCTGCGTTGATAC860.22046194467943295No Hit
TCGCTATGGTGTTCACGTACTCTGCGTTGATAC810.20764438975621008No Hit
CAGCTATGGTGTTCACGTACTCTGCGTTGATAC810.20764438975621008No Hit
ATGCTATGGTGTTCACGTACTCTGCGTTGATAC800.20508087877156553No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA790.20251736778692095No Hit
GTGAATGGGCTATGGAAAAAAAAAAAAAAAAAA750.1922633238483427No Hit
GTGAATGGGCTATGGTAAAAAAAAAAAAAAAAA730.18713630187905356No Hit
GGGCTATGGTGTTCACGTACTCTGCGTTGATAC730.18713630187905356No Hit
GCTATGGTGTAAAAAAAAAAAAAAAAAAAAAAA720.18457279089440898No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT700.17944576892511985No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA700.17944576892511985No Hit
GTGAATGGGTGTTCACGTACTCAAAAAAAAAAA700.17944576892511985No Hit
GAGCTATGGTGTTCACGTACTCTGCGTTGATAC680.17431874695583072No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA680.17431874695583072No Hit
GGCTATGGTGTTCACGTACTCTGCGTTGATACC680.17431874695583072No Hit
GCTATGGTGAAAAAAAAAAAAAAAAAAAAAAAA660.16919172498654156No Hit
CGGCTATGGTGTTCACGTACTCTGCGTTGATAC650.166628214001897No Hit
AACGCAGAGTGAATGGGCTATGGTGTAAAAAAA640.16406470301725243No Hit
GAGTACGTGAACACCAAAAAAAAAAAAAAAAAA640.16406470301725243No Hit
GGCTATGGTGAAAAAAAAAAAAAAAAAAAAAAA610.15637417006331872No Hit
GAGTGAATGGGCTATGGTGTAAAAAAAAAAAAA610.15637417006331872No Hit
GTGAATGGGTGTTCACGTACTCTGAAAAAAAAA590.1512471480940296No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA580.148683637109385No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA570.14612012612474046No Hit
GTATCAACGCAGAGTGAATGGGCTATGGTGAAA560.14355661514009588No Hit
GTTCACGTACTCTGCAAAAAAAAAAAAAAAAAA540.13842959317080672No Hit
GTGAATGGGGCTATGGTGTAAAAAAAAAAAAAA530.13586608218616217No Hit
GAATGGGGCTATGGTGAAAAAAAAAAAAAAAAA510.13073906021687304No Hit
ACGCAGAGTGAATGGGCTATGGTAAAAAAAAAA510.13073906021687304No Hit
TATCAACGCAGAGTGAATGGGTGTTCACGTACT500.12817554923222846No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA500.12817554923222846No Hit
GTGAATGGGGCTATGGTAAAAAAAAAAAAAAAA500.12817554923222846No Hit
GCTATGGTGTTCACGTACTCAAAAAAAAAAAAA490.12561203824758388No Hit
GAATGGGCTATGGTGAAAAAAAAAAAAAAAAAA480.12304852726293931No Hit
GAGTGAATGGGGCTATGGTGAAAAAAAAAAAAA480.12304852726293931No Hit
CGCTATGGTGTTCACGTACTCTGCGTTGATACC470.12048501627829475No Hit
AAGCTATGGTGTTCACGTACTCTGCGTTGATAC460.1179215052936502No Hit
GAATGGGCTATGGTGTAAAAAAAAAAAAAAAAA450.11535799430900562No Hit
ATCAACGCAGAGTGAATGGGCTATGGTGAAAAA440.11279448332436105No Hit
GTATCATCGCAGAGTGAATGGGCTATGGTGAAA430.11023097233971647No Hit
TTGCTATGGTGTTCACGTACTCTGCGTTGATAC430.11023097233971647No Hit
ACGCAGAGTGAATGGGGCTATGGTGAAAAAAAA410.10510395037042733No Hit
TATCAACGCAGAGTGAATGGGCTATGGTGTTCA410.10510395037042733No Hit
TATCAACGCAGAGTGAATGGGGCTATGGTGAAA410.10510395037042733No Hit
CCATAGCAAAAAAAAAAAAAAAAAAAAAAAAAA400.10254043938578276No Hit
GTGAATGGGGCTATGGAAAAAAAAAAAAAAAAA400.10254043938578276No Hit
ACGCAGAGTGAATGGGCTATGGAAAAAAAAAAA400.10254043938578276No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACCCC200.00851055927.01
CAACTCA200.00851055927.01
GTATCAT200.00851055927.01
AACTCAG200.00851055927.02
TATCATC200.00851055927.02
CTACGCA401.7948481E-523.6254
ATCTACG351.9903526E-423.1428592
TCTACGC351.9903526E-423.1428593
TACGCAG351.9903526E-423.1428595
TATCTAC351.9903526E-423.1428591
GATACCA707.410563E-921.21428727
TGATACC803.4404366E-818.562526
TTGATAC2400.018.562527
GTTGATA2300.018.19565226
CGTTGAT2300.018.19565225
TATCAAC2650.017.3207551
ATCAACG3100.014.8064512
TCAACGC3200.014.343753
GCGTTGA3000.013.9524
TCTGCGT10050.013.1641827
Produced by FastQC (version 0.11.2)