FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_63.35200000060105.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_63.35200000060105.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41291
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA3310.8016274732992662No Hit
CAGCTATGCCAACGTACTCTGCGTTGATACCAC3270.7919401322322056No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA1900.4601487006853794No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA1840.44561768908478844No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1790.4335085127509627No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT1680.40686832481654595No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA1660.40202465428301565No Hit
CCCCTCAGCTATGCCAACGTACTCTGCGTTGAT1550.37538446634859896No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA1440.34874427841418226No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA1270.3075730788791746No Hit
GTGAATGGGTCAGCTATGCCAAAAAAAAAAAAA1270.3075730788791746No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA1230.297885737812114No Hit
GAGTACGTTGGCAAAAAAAAAAAAAAAAAAAAA840.20343416240827297No Hit
ATGCCAACGTACTCAAAAAAAAAAAAAAAAAAA760.18405948027415175No Hit
CCCTTCAGCTATGCCAACGTACTCTGCGTTGAT710.17195030394032598No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.16952846867356083No Hit
GCTATGCCAAAAAAAAAAAAAAAAAAAAAAAAA640.15499745707296989No Hit
GCTATGCCAACGTACTCAAAAAAAAAAAAAAAA630.15257562180620474No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.1501537865394396No Hit
CCTCAGCTATGCCAACGTACTCTGCGTTGATAC610.1477319512726744No Hit
CCCTCAGCTATGCCAACGTACTCTGCGTTGATA570.13804461020561382No Hit
ACTCTAACTATGCCAACGTACTCAAAAAAAAAA570.13804461020561382No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA550.13320093967208352No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT530.1283572691385532No Hit
CCTCTCAGCTATGCCAACGTACTCTGCGTTGAT530.1283572691385532No Hit
CCCCCTCAGCTATGCCAACGTACTCTGCGTTGA520.12593543387178804No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA520.12593543387178804No Hit
CCCGTCAGCTATGCCAACGTACTCTGCGTTGAT520.12593543387178804No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA510.1235135986050229No Hit
GCCCTCAGCTATGCCAACGTACTCTGCGTTGAT490.11866992807149257No Hit
CCCATCAGCTATGCCAACGTACTCTGCGTTGAT470.11382625753796227No Hit
ACGCAGAGTGAATGGGTCAGCTATGCCAAAAAA460.11140442227119711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTACG455.3523763E-827.0073092
GTATCAT356.3682946E-627.0073091
TCTACGC455.3523763E-827.0073093
TACGCAG455.3523763E-827.0073095
TATCTAC455.3523763E-827.0073091
CTACGCA455.3523763E-827.0073094
GTAGAGT200.00850446527.0073074
AACGTAG200.00850446527.0073071
TAGAGTG200.00850446527.0073075
TATCATC401.7940893E-523.6313932
ATCATCG454.4561006E-521.0056843
TCATCGC454.4561006E-521.0056844
CATCGCA454.4561006E-521.0056845
ATCGCAG501.0115443E-418.8821186
TATCAAC3650.017.758231
TCGCAGA552.0989915E-417.1655627
CGTTGAT2350.016.66408327
ATCAACG4150.015.9440742
TCTGCGT11500.015.73469327
TCAACGC4400.015.0381613
Produced by FastQC (version 0.11.2)