FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_62.352000000600ea.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_62.352000000600ea.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38048
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA12413.261669470142977No Hit
CAACAGAGCATACGTACTCTGCGTTGATACCAC3821.003994953742641No Hit
GCATACGTACTCAAAAAAAAAAAAAAAAAAAAA3270.8594407064760303No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA2650.696488645920942No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA2500.6570647603027754No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA2430.6386669470142978No Hit
GAGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2140.5624474348191757No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCAAA2140.5624474348191757No Hit
CAACAGAGCATACGTACTCAAAAAAAAAAAAAA1960.5151387720773759No Hit
ACGCAGAGTGAATGGGCAACAGAGCATACGTAC1770.465201850294365No Hit
GAGCATAAAAAAAAAAAAAAAAAAAAAAAAAAA1570.41263666947014294No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1380.36269974768713203No Hit
AACGCAGAGTGAATGGGCAACAGAGCAAAAAAA1380.36269974768713203No Hit
CAACAGAGCAAAAAAAAAAAAAAAAAAAAAAAA1220.3206476030277544No Hit
GAGCATACGTACAAAAAAAAAAAAAAAAAAAAA1190.31276282590412113No Hit
GTGAATGGGGCAACAGAGCAAAAAAAAAAAAAA1160.3048780487804878No Hit
GTGAATGGGCAACAGAGAAAAAAAAAAAAAAAA1090.28648023549201007No Hit
GAGTGAATGGGCAACAGAGCAAAAAAAAAAAAA1080.28385197645079896No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA1070.28122371740958785No Hit
GTGAATGGGCAACAGAGCATACGTACTCAAAAA1050.2759671993271657No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAT1050.2759671993271657No Hit
GCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1040.27333894028595457No Hit
AACGCAGAGTGAATGGGCAACAGAGCATACGTA1020.2680824222035324No Hit
ACGCAGAGTGAATGGGCAACAGAGAAAAAAAAA1000.2628259041211102No Hit
GGGCAACAGAGCAAAAAAAAAAAAAAAAAAAAA980.25756938603868795No Hit
GAGCATACGTAAAAAAAAAAAAAAAAAAAAAAA970.2549411269974769No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.2523128679562658No Hit
ACGCAGAGTGAATGGGCAACAGAGCATAAAAAA960.2523128679562658No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGAAAA950.24968460891505467No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT940.2470563498738436No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAT850.22340201850294364No Hit
GTGAATGGGCAACAGAGCATAAAAAAAAAAAAA840.22077375946173255No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT780.20500420521446594No Hit
TACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA740.19449116904962152No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC740.19449116904962152No Hit
GGGCAACAGAGCATAAAAAAAAAAAAAAAAAAA710.1866063919259882No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1866063919259882No Hit
AACGCAGAGTGAATGGGCAACAGAGAAAAAAAA700.18397813288477713No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA680.17872161480235493No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGCAA660.17346509671993274No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA660.17346509671993274No Hit
GGGCAACAGAGCATACGTACTCAAAAAAAAAAA650.1708368376787216No Hit
CAACTCAGAGTGAATGGGCAACAGAGCAAAAAA590.155067283431455No Hit
GTGAATGGGGCAACAGAGAAAAAAAAAAAAAAA570.14981076534903282No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCATA570.14981076534903282No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA570.14981076534903282No Hit
CAACAGAGCATAAAAAAAAAAAAAAAAAAAAAA560.1471825063078217No Hit
GTGAATGGGCAACAGAAAAAAAAAAAAAAAAAA560.1471825063078217No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAA550.1445542472666106No Hit
TATCAACGCAGAGTGAATGGGGCAACAGAGCAT550.1445542472666106No Hit
GAGTGAATGGGCAACAGAGAAAAAAAAAAAAAA550.1445542472666106No Hit
CATACGTACTCAAAAAAAAAAAAAAAAAAAAAA550.1445542472666106No Hit
CAACAGAGCATACGTAAAAAAAAAAAAAAAAAA540.14192598822539948No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA540.14192598822539948No Hit
ACGCAGAGTGAATGGGCAACAGAAAAAAAAAAA530.1392977291841884No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCATAC530.1392977291841884No Hit
ATCAACGCAGAGTGAATGGGCAACAGAGCAAAA520.13666947014297728No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA520.13666947014297728No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA510.1340412111017662No Hit
AACGCAGAGTGAATGGGCAACAGAGCATAAAAA510.1340412111017662No Hit
GGTATCAACGCAGAGTGAATGGGCAACAGAGCA510.1340412111017662No Hit
GAGTGAATGGGGCAACAGAGCAAAAAAAAAAAA510.1340412111017662No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA490.12878469301934398No Hit
CAACAGAGCATACGTACAAAAAAAAAAAAAAAA480.1261564339781329No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA480.1261564339781329No Hit
GAGTGAATGGGCAACAGAGCATACGTACTCAAA480.1261564339781329No Hit
ATGGGCAACAGAGCAAAAAAAAAAAAAAAAAAA470.1235281749369218No Hit
TATCTACGCAGAGTGAATGGGCAACAGAGCAAA460.1208999158957107No Hit
ACAGAGCATACGTACTCAAAAAAAAAAAAAAAA450.11827165685449957No Hit
GTGAATGGGGCAACAGAGCATACGTACTCAAAA440.11564339781328847No Hit
GAGCATACAAAAAAAAAAAAAAAAAAAAAAAAA440.11564339781328847No Hit
GTATCATCGCAGAGTGAATGGGCAACAGAGCAA440.11564339781328847No Hit
GAGCATACGTACTAAAAAAAAAAAAAAAAAAAA430.11301513877207738No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC430.11301513877207738No Hit
GGGCAACAGAGAAAAAAAAAAAAAAAAAAAAAA420.11038687973086628No Hit
GCATACGTACAAAAAAAAAAAAAAAAAAAAAAA410.10775862068965517No Hit
GTATCAACGCAGAGTGAATGGGCAACAGAGAAA410.10775862068965517No Hit
GTGAATGGGGCAACAGAAAAAAAAAAAAAAAAA400.10513036164844407No Hit
TATCAACGCAGAGTGAATGGGCAACAGAAAAAA400.10513036164844407No Hit
CCCTGCAACAGAGCATACGTACTCTGCGTTGAT390.10250210260723297No Hit
GAGTGAATGGGCAACAGAGCATAAAAAAAAAAA390.10250210260723297No Hit
TACGTACTCTGCGTTGATACCAAAAAAAAAAAA390.10250210260723297No Hit
ACGCAGAGTGAATGGGGCAACAGAGCAAAAAAA390.10250210260723297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCATC653.6379788E-1226.9986842
GTATCAT700.026.9986821
CAACTCA653.1450327E-922.8450411
TCATCGC851.4370016E-1020.6460514
TCGCAGA801.537046E-920.2490127
ATCGCAG801.537046E-920.2490126
CATCGCA801.537046E-920.2490125
ATCATCG903.110472E-1019.499053
TGAAAGG350.005438431619.28477315
ATCTACG501.0020032E-418.8990782
TATCTAC501.0020032E-418.8990781
AACTCAG803.4349796E-818.5615942
TATCAAC3050.018.1466541
GAAAGGG450.001083926217.99912316
ACTCAGA856.875598E-817.4697363
TCAGAGT856.875598E-817.4697365
CTCAGAG856.875598E-817.4697364
TCTACGC552.0793066E-417.180983
ATACCAC653.862245E-516.63649427
GGTATCA707.365261E-515.4278181
Produced by FastQC (version 0.11.2)