FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_61.352000000600c0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_61.352000000600c0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30486
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGAGTCAAAAAAAAAAAAAAAAAAAAAAA3481.1415075772485732No Hit
CCACCATAGAGTCACGTACTCTGCGTTGATACC2190.7183625270616021No Hit
CCATAGAGAAAAAAAAAAAAAAAAAAAAAAAAA1970.6461982549366923No Hit
CCATAGAGTCACGTACTCAAAAAAAAAAAAAAA1820.5969953421242538No Hit
ACCATAGAGTCACGTACTCTGCGTTGATACCAC1620.5313914583743358No Hit
CCATAGAGTAAAAAAAAAAAAAAAAAAAAAAAA1570.5149904874368563No Hit
GCACCATAGAGTCAAAAAAAAAAAAAAAAAAAA1370.4493866036869383No Hit
GCACCATAGAGTCACGTACTCTGCGTTGATACC1220.4001836908744998No Hit
ACACCATAGAGTCACGTACTCTGCGTTGATACC1080.35426097224955716No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA960.3148986419996064No Hit
GCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA890.2919372826871351No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTC780.2558551466246802No Hit
CCATAGAAAAAAAAAAAAAAAAAAAAAAAAAAA780.2558551466246802No Hit
GAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAAA700.22961359312471297No Hit
TCACCATAGAGTCACGTACTCTGCGTTGATACC680.22305320474972118No Hit
GCACCATAGAGTAAAAAAAAAAAAAAAAAAAAA680.22305320474972118No Hit
GAGTGAATGGGCACCATAGAGTCAAAAAAAAAA630.20665223381224168No Hit
ACGCAGAGTGAATGGGCACCATAGAGTCAAAAA630.20665223381224168No Hit
CCATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA580.19025126287476218No Hit
GTGAATGGGCACCATAGAGTCAAAAAAAAAAAA520.1705700977497868No Hit
GAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.1705700977497868No Hit
ACTCTATGGTGAAAAAAAAAAAAAAAAAAAAAA500.16400970937479498No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA490.16072951518729908No Hit
ACTCTATGGTGCAAAAAAAAAAAAAAAAAAAAA470.1541691268123073No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA460.15088893262481137No Hit
GTACGTGACTCTAAAAAAAAAAAAAAAAAAAAA460.15088893262481137No Hit
ACGCAGAGTGAATGGGCACCATAGAGAAAAAAA460.15088893262481137No Hit
ACGCAGAGTGAATGGGCACCATAGAAAAAAAAA460.15088893262481137No Hit
GAGTACGTGACTCTAAAAAAAAAAAAAAAAAAA450.14760873843731548No Hit
CACCATAGAGTCACGTACTCTGCGTTGATACCA440.14432854424981958No Hit
ACACCATAGAGTCAAAAAAAAAAAAAAAAAAAA440.14432854424981958No Hit
GAGTGAATGGGCACCATAGAGTAAAAAAAAAAA440.14432854424981958No Hit
GTGAATGGGCACCATAGAGAAAAAAAAAAAAAA420.1377681558748278No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGAA420.1377681558748278No Hit
GTGAATGGGCACCATAGAGTAAAAAAAAAAAAA410.1344879616873319No Hit
GTGAATGGGACACCATAGAGAAAAAAAAAAAAA410.1344879616873319No Hit
CCACCATAGAGAAAAAAAAAAAAAAAAAAAAAA400.131207767499836No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA390.1279275733123401No Hit
GAGTGAATGGGCACCATAGAGAAAAAAAAAAAA390.1279275733123401No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA390.1279275733123401No Hit
GTATCAACGCAGAGTGAATGGGCACCATAGAGT380.1246473791248442No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAAAA370.1213671849373483No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA370.1213671849373483No Hit
GTGAATGGGACACCATAGAGTCAAAAAAAAAAA370.1213671849373483No Hit
GTGAATGGGGCACCATAAAAAAAAAAAAAAAAA360.1180869907498524No Hit
ACACCATAGAGAAAAAAAAAAAAAAAAAAAAAA360.1180869907498524No Hit
ATCAACGCAGAGTGAATGGGCACCATAGAGTCA350.11480679656235648No Hit
GTGAATGGGACACCATAGAAAAAAAAAAAAAAA350.11480679656235648No Hit
CCATAGAGTCACAAAAAAAAAAAAAAAAAAAAA350.11480679656235648No Hit
GAGTGAATGGGCACCATAGAAAAAAAAAAAAAA340.11152660237486059No Hit
GTGAATGGGCACCATAGAAAAAAAAAAAAAAAA340.11152660237486059No Hit
TATCAACGCAGAGTGAATGGGCACCATAGAGTA340.11152660237486059No Hit
GTGAATGGGGCACCATAGAGTCAAAAAAAAAAA340.11152660237486059No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA320.1049662139998688No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCTGA200.00848169827.01
TATCATC257.653196E-427.02
GTATCAT300.002214752822.51
ATCCACC350.00541264119.2857157
TCGCAGA350.00541264119.2857157
ATCATCG350.00541264119.2857153
ATCGCAG350.00541264119.2857156
TCATCGC350.00541264119.2857154
CATCGCA350.00541264119.2857155
TATCAAC1650.018.01
ATCAACG1800.015.752
TGATACC1054.351932E-815.42857327
TTGATAC1054.351932E-815.42857326
GTATCAA1051.1742815E-512.8571431
TCAACGC2003.6379788E-1212.8250013
TCTGCGT9300.012.77419327
TGCGTTG3100.012.62903227
GTCACCA750.002165688212.67
GTTGATA1359.335108E-712.025
ACTCTAT2057.094059E-1111.8536581
Produced by FastQC (version 0.11.2)