FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_60.352000000600a7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_60.352000000600a7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44228
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4681.058153206113774No Hit
CACGTACTCTGCGAAAAAAAAAAAAAAAAAAAA4591.0378041059962015No Hit
GTGAATGGGCGTAAGGATAAAAAAAAAAAAAAA4230.9564077055259113No Hit
CACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA4200.9496246721533871No Hit
GGATTCACGTACTCAAAAAAAAAAAAAAAAAAA3630.8207470380754274No Hit
GCGTAAGGATTCACGTACTCTGCGTTGATACCA3440.7777878267161075No Hit
GTGAATGGGCGTAAGGAAAAAAAAAAAAAAAAA2940.6647372705073709No Hit
TATCAACGCAGAGTGAATGGGATTCACGTACTC2610.5901239034096049No Hit
ACGCAGAGTGAATGGGCGTAAGGATAAAAAAAA2410.5449036809261102No Hit
GCGTAAGGATAAAAAAAAAAAAAAAAAAAAAAA2370.5358596364294113No Hit
GTGAATGGGGCGTAAGGATAAAAAAAAAAAAAA2160.48837840282174194No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATAAA1970.445419191462422No Hit
GTGAATGGGGCGTAAGGAAAAAAAAAAAAAAAA1900.42959211359319893No Hit
ATTCACGTACTCTGCGAAAAAAAAAAAAAAAAA1760.39793795785475267No Hit
GAGTGAATGGGCGTAAGGATAAAAAAAAAAAAA1700.38437189110970427No Hit
ACGCAGAGTGAATGGGCGTAAGGAAAAAAAAAA1600.36176177986795693No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGAAAAA1470.33236863525368543No Hit
TTCACGTACTCTGCGAAAAAAAAAAAAAAAAAA1330.3007144795152392No Hit
GTATCAACGCAGAGTGAATGGGATTCACGTACT1270.2871484127701908No Hit
AACGCAGAGTGAATGGGCGTAAGGATAAAAAAA1200.2713213349009677No Hit
GAGTGAATGGGCGTAAGGAAAAAAAAAAAAAAA1160.2622772904042688No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1080.24418920141087097No Hit
GAGTGAATGGGGCGTAAGGATAAAAAAAAAAAA1060.2396671791625215No Hit
GATTCACGTACTCAAAAAAAAAAAAAAAAAAAA1040.23514515691417204No Hit
GTGAATGGGATTCACGTACTCTGCGAAAAAAAA1020.23062313466582257No Hit
GGCGTAAGGATAAAAAAAAAAAAAAAAAAAAAA990.22384010129329837No Hit
GAGTGAATGGGATTCACGTACTCTGCGAAAAAA970.2193180790449489No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.21027403454825No Hit
GATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA900.2034910011757258No Hit
AACGCAGAGTGAATGGGCGTAAGGAAAAAAAAA890.20122999005155107No Hit
GGATTCACGTACTCTGCGAAAAAAAAAAAAAAA880.19896897892737633No Hit
GATTCACGTACTCTGCGAAAAAAAAAAAAAAAA870.1967079678032016No Hit
AACGCAGAGTGAATGGGATTCACGTACTCTGCG840.1899249344306774No Hit
CACGTACTCTGCAAAAAAAAAAAAAAAAAAAAA830.18766392330650267No Hit
GTGAATGGGCGTAAGGATTCACGTACTCAAAAA810.1831419010581532No Hit
GCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAAA810.1831419010581532No Hit
CGTAAGGATTCACGTACTCTGCGTTGATACCAC780.17635886768562903No Hit
CTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA770.1740978565614543No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGA760.17183684543727956No Hit
GGCGTAAGGAAAAAAAAAAAAAAAAAAAAAAAA740.1673148231889301No Hit
GAGTGAATGGGGCGTAAGGAAAAAAAAAAAAAA730.16505381206475536No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGATAA730.16505381206475536No Hit
TATCAACGCAGAGTGAATGGGCGTAAGGATTCA720.16279280094058063No Hit
ATTCACGTACTCTGCGTAAAAAAAAAAAAAAAA720.16279280094058063No Hit
GCGTAAGGATTCACGTACTCAAAAAAAAAAAAA690.15600976756805643No Hit
ATCAACGCAGAGTGAATGGGATTCACGTACTCT680.1537487564438817No Hit
ACGCAGAGTGAATGGGGCGTAAGGAAAAAAAAA680.1537487564438817No Hit
GTATCAACGCAGAGTGAATGGGCGTAAGGAAAA670.15148774531970696No Hit
ACGCAGAGTGAATGGGGCGTAAGGATAAAAAAA670.15148774531970696No Hit
GAATGGGGCGTAAGGATAAAAAAAAAAAAAAAA670.15148774531970696No Hit
ATCAACGCAGAGTGAATGGGCGTAAGGATAAAA660.14922673419553223No Hit
GAATGGGCGTAAGGATAAAAAAAAAAAAAAAAA660.14922673419553223No Hit
GGATTCAAAAAAAAAAAAAAAAAAAAAAAAAAA640.14470471194718276No Hit
GCGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.14018268969883332No Hit
ACGCAGAGTGAATGGGCGTAAGGATTCACGTAC610.1379216785746586No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGATAA610.1379216785746586No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA600.13566066745048386No Hit
TATCAACGCAGAGTGAATGGGGCGTAAGGAAAA600.13566066745048386No Hit
GAGTGAATGGGATTCACGTACTCTGCGTAAAAA590.13339965632630912No Hit
GTGAATGGGGGCGTAAGGATAAAAAAAAAAAAA580.1311386452021344No Hit
ACGCAGAGTGAATGGGATTCACGTACTCTGCGT580.1311386452021344No Hit
GTGAATGGGATTCACGTACTCTGCGTAAAAAAA580.1311386452021344No Hit
CAACTCAGAGTGAATGGGCGTAAGGATAAAAAA580.1311386452021344No Hit
GTGAATGGGCGTAAAAAAAAAAAAAAAAAAAAA540.12209460070543549No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA530.11983358958126075No Hit
CGTAAGGATAAAAAAAAAAAAAAAAAAAAAAAA530.11983358958126075No Hit
GTGAATGGGAGCGTAAGGATAAAAAAAAAAAAA520.11757257845708602No Hit
GTATCATCGCAGAGTGAATGGGCGTAAGGATAA510.11531156733291129No Hit
GGATTCACGTACAAAAAAAAAAAAAAAAAAAAA500.11305055620873655No Hit
TTCACGTACTCTGCGTAAAAAAAAAAAAAAAAA500.11305055620873655No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA490.11078954508456182No Hit
GTGAATGGGAGCGTAAGGAAAAAAAAAAAAAAA480.10852853396038709No Hit
GGATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA470.10626752283621237No Hit
GGATTCACGTACTCTGCGTAAAAAAAAAAAAAA470.10626752283621237No Hit
GAATGGGCGTAAGGAAAAAAAAAAAAAAAAAAA470.10626752283621237No Hit
TATCTACGCAGAGTGAATGGGCGTAAGGATAAA450.1017455005878629No Hit
GTGAATGGGGGCGTAAGGAAAAAAAAAAAAAAA450.1017455005878629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG200.00852256927.02
CTGAGTG200.00852256927.04
ACGCTGA200.00852256927.01
GTATCTA200.00852256927.01
GTATCAT455.3858457E-827.01
GCTGAGT200.00852256927.03
TGAGTGA200.00852256927.05
CAACTCA651.1641532E-1024.9230791
TATCATC501.4942088E-724.3000012
AACTCAG702.928573E-1023.1428592
TCGCAGA553.749028E-722.090917
TAGAGTG553.749028E-722.090915
ATCATCG553.749028E-722.090913
ATCGCAG553.749028E-722.090916
TCATCGC553.749028E-722.090914
GTAGAGT504.0883297E-621.64
ACGTAGA504.0883297E-621.62
CGTAGAG504.0883297E-621.63
ACTCAGA756.9667294E-1021.5999983
TCAGAGT756.9667294E-1021.5999985
Produced by FastQC (version 0.11.2)