FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_58.35200000060063.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_58.35200000060063.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62173
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGCAGAGACGTACGTACTCTGCGTTGATACCA6251.0052595177971146No Hit
CGTACGTACTCAAAAAAAAAAAAAAAAAAAAAA5970.9602238913998038No Hit
GTGAATGGGGAGACGTACGTACTCAAAAAAAAA4250.6835764721020379No Hit
GACGTACGTACTCAAAAAAAAAAAAAAAAAAAA3450.5549032538240072No Hit
TATCAACGCAGAGTGAATGGGGAGACGTACGTA2840.4567899248870088No Hit
GAGACGTACGTACTCAAAAAAAAAAAAAAAAAA2560.4117542984896981No Hit
GTGAATGGGAGACGTACGTACTCAAAAAAAAAA2560.4117542984896981No Hit
GCAGAGACGTACGTACTCTGCGTTGATACCACT2340.3763691634632397No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA2170.34902610457915817No Hit
ACGCAGAGTGAATGGGGAGACGTACGTACTCAA2150.3458092741222074No Hit
GACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1830.29433998681099516No Hit
GAGTGAATGGGGAGACGTACGTACTCAAAAAAA1720.27664741929776593No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGT1600.2573464365560613No Hit
ATGCAGAGACGAAAAAAAAAAAAAAAAAAAAAA1470.23643703858588133No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA1450.23322020812893057No Hit
GAGACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1440.2316117929004552No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1380.2219613015296029No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1160.18657616650314446No Hit
AACGCAGAGTGAATGGGGAGACGTACGTACTCA1160.18657616650314446No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1140.1833593360461937No Hit
ATGCAGAGACGTACGAAAAAAAAAAAAAAAAAA1130.18175092081771832No Hit
ATGCAGAGACGTACAAAAAAAAAAAAAAAAAAA1090.17531725990381677No Hit
GTATCAACGCAGAGTGAATGGGGAGACGTACGT1040.16727518376143985No Hit
ATGCAGAGACGTAAAAAAAAAAAAAAAAAAAAA1000.1608415228475383No Hit
GTATCAACGCAGAGTGAATGGGATGCAGAGACG960.1544078619336368No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA950.1527994467051614No Hit
GAGTGAATGGGATGCAGAGACGAAAAAAAAAAA950.1527994467051614No Hit
GAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA930.14958261624821062No Hit
TATCAACGCAGAGTGAATGGGAGACGTACGTAC880.14154054010583372No Hit
GAATGGGGAGACGTACGTACTCAAAAAAAAAAA870.13993212487735834No Hit
CCCCCATGCAGAGACGTACGTACTCTGCGTTGA840.1351068791919322No Hit
TATCAACGCAGAGTGAATGGGATGCAGAGACGA810.13028163350650604No Hit
TATCAACGCAGAGTGAATGGGAGAGACGTACGT760.12223955736412911No Hit
GTGAATGGGATGCAGAGACGAAAAAAAAAAAAA750.12063114213565374No Hit
GTATCATCGCAGAGTGAATGGGATGCAGAGACG730.11741431167870298No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA720.1158058964502276No Hit
ACGCAGAGTGAATGGGATGCAGAGACGAAAAAA720.1158058964502276No Hit
ATGCAGAGACAAAAAAAAAAAAAAAAAAAAAAA720.1158058964502276No Hit
CGTACGTACTCTAAAAAAAAAAAAAAAAAAAAA720.1158058964502276No Hit
GACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA710.1141974812217522No Hit
GGAGACGTACGTACTCAAAAAAAAAAAAAAAAA690.11098065076480144No Hit
ATCAACGCAGAGTGAATGGGGAGACGTACGTAC670.10776382030785069No Hit
GTGAATGGGATGCAGAGACAAAAAAAAAAAAAA660.10615540507937528No Hit
GTGAATGGGAGAGACGTACGTACTCAAAAAAAA650.1045469898508999No Hit
GAGACGAAAAAAAAAAAAAAAAAAAAAAAAAAA640.10293857462242452No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCAATG200.00854884327.02
GTATCAT1000.025.651
TATCATC1000.025.652
CAACTCA1200.024.751
CTCAGAG1200.024.754
TCAGAGT1150.024.6521745
CATCGCA1100.024.5454545
ATCATCG1050.024.4285743
TCATCGC1050.024.4285744
AACTCAG1250.023.762
ACTCAGA1250.023.763
TCGCAGA1150.023.478267
ATCGCAG1150.023.478266
ATCTACG950.022.7368432
TCTACGC950.022.7368433
TACGCAG950.022.7368435
TATCTAC950.022.7368431
CTACGCA950.022.7368434
GCCAATG350.005475305519.2857153
GGTATCA1101.2732926E-1118.4090921
Produced by FastQC (version 0.11.2)