FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_57.3520000006004a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_57.3520000006004a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34329
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTACGTACCGTAACGTACTCTGCGTTGATACC4661.3574528824026333No Hit
GCTACGTACCGTAACGTACTCTGCGTTGATACC1940.565119869498092No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1890.550554924407935No Hit
GCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA1720.5010341111014011No Hit
GCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA1700.4952081330653384No Hit
ACTACGTACCGTAACGTACTCTGCGTTGATACC1540.44860030877683593No Hit
CTACGTACCGTAACGTACTCTGCGTTGATACCA1500.43694835270471033No Hit
TCTACGTACCGTAACGTACTCTGCGTTGATACC1250.3641236272539252No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA1030.30003786885723444No Hit
GTGAATGGGCTACGTACCGAAAAAAAAAAAAAA940.2738209676949518No Hit
CCCCTACGTACCGTAACGTACTCTGCGTTGATA870.25343004456873197No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA840.24469107751463776No Hit
CCTACGTACCGAAAAAAAAAAAAAAAAAAAAAA830.24177808849660637No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT830.24177808849660637No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA820.23886509947857498No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA780.22721314340644938No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA730.21264819831629234No Hit
CCCTACGTACCGTAACGTACTCTGCGTTGATAC700.20390923126219815No Hit
GTGAATGGGCTACGTACCGTAAAAAAAAAAAAA690.20099624224416673No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA610.17769233009991553No Hit
ACTACGTACCGAAAAAAAAAAAAAAAAAAAAAA590.1718663520638527No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGAA580.16895336304582131No Hit
GTGAATGGGCTACGTACAAAAAAAAAAAAAAAA580.16895336304582131No Hit
CCTACGTACCGTAAAAAAAAAAAAAAAAAAAAA570.1660403740277899No Hit
GCTACGTACAAAAAAAAAAAAAAAAAAAAAAAA540.1573014069736957No Hit
ACTACGTACCGTAAAAAAAAAAAAAAAAAAAAA530.15438841795566433No Hit
CTCTACGTACCGTAACGTACTCTGCGTTGATAC510.1485624399196015No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA500.14564945090157008No Hit
ACGCAGAGTGAATGGGCTACGTACCGAAAAAAA480.1398234728655073No Hit
GCCCTACGTACCGTAACGTACTCTGCGTTGATA460.1339974948294445No Hit
GAGTGAATGGGCTACGTACCGAAAAAAAAAAAA430.12525852777535026No Hit
GCCTACGTACCGTAACGTACTCTGCGTTGATAC420.12234553875731888No Hit
GTGAATGGGGCTACGTACCGAAAAAAAAAAAAA400.11651956072125608No Hit
AACGCAGAGTGAATGGGCTACGTACCGAAAAAA380.11069358268519328No Hit
TATCAACGCAGAGTGAATGGGCTACGTACCGTA350.10195461563109907No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCTA200.00849870426.998546
ATGGGAG501.4746729E-724.2986895
GACCTAC300.002221157822.4987837
TGATACC1700.021.4713227
TTGATAC2200.016.56728726
GTTGATA2650.013.75397525
CGTTGAT2650.013.75397524
ACCTACG1602.2737368E-1013.499278
TCTGCGT9600.013.0965127
TTCTACG956.7140645E-512.7887847
TATCAAC1603.3542165E-912.6555661
GCGTTGA3100.011.75742923
CACTACG1051.7098612E-411.5708047
CTCTACG1351.256868E-510.9994067
CCGTAAA2007.707058E-910.79941759
ATGGGGT1301.05183004E-410.3840555
AATGGGA3005.456968E-129.8994664
CGCTACG1100.00329773039.8176528
ATCAACG2001.0064541E-69.4494912
TGGGGTA1150.0047618059.3907986
Produced by FastQC (version 0.11.2)