FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_56.35200000060020.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_56.35200000060020.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences36187
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATGAGCACTGTACGTACTCTGCGTTGATACCA11963.3050543012684113No Hit
GCACTGTACGTACAAAAAAAAAAAAAAAAAAAA5391.4894851742338409No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAC3681.016939785005665No Hit
GTGAATGGGCACTGTACGTACAAAAAAAAAAAA3440.9506176251139913No Hit
GCACTGTACGTACTCAAAAAAAAAAAAAAAAAA2800.7737585320695277No Hit
ACGCAGAGTGAATGGGCACTGTACGTACAAAAA2710.7488877221101501No Hit
CTGTACGTACAAAAAAAAAAAAAAAAAAAAAAA2540.7019095255202145No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA2380.6576947522590986No Hit
GTACGTACAAAAAAAAAAAAAAAAAAAAAAAAA1830.5057064691740127No Hit
GAGTGAATGGGCACTGTACGTACAAAAAAAAAA1830.5057064691740127No Hit
CCCATGAGCACTGTACGTACTCTGCGTTGATAC1810.5001796225163733No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1740.48083565921463506No Hit
GTATCAACGCAGAGTGAATGGGCACTGTACGTA1710.4725453892281759No Hit
CTGTACGTACTCAAAAAAAAAAAAAAAAAAAAA1610.4449111559399785No Hit
GTGAATGGGCACTGTACGTACTCAAAAAAAAAA1210.33437422278718876No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1190.3288473761295493No Hit
AACGCAGAGTGAATGGGCACTGTACGTACAAAA1150.3177936828142703No Hit
GAATGGGCACTGTACGTACAAAAAAAAAAAAAA1130.31226683615663087No Hit
CCATGAGCACTGTACGTACTCTGCGTTGATACC1080.29844971951253213No Hit
GTATCATCGCAGAGTGAATGGGCACTGTACGTA1040.28739602619725313No Hit
GTGAATGGGCACTGTACGTAAAAAAAAAAAAAA970.268052062895515No Hit
ACGCAGAGTGAATGGGCACTGTACGTACTCAAA850.23489098294967806No Hit
GCCATGAGCACTGTACGTACTCTGCGTTGATAC760.21002017299030037No Hit
GTACTACTCAAAAAAAAAAAAAAAAAAAAAAAA730.20172990300384117No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACA710.19620305634620167No Hit
GTACTACAAAAAAAAAAAAAAAAAAAAAAAAAA680.18791278635974246No Hit
GGTATCAACGCAGAGTGAATGGGCACTGTACGT680.18791278635974246No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA670.1851493630309227No Hit
GTACGTACCTAAAAAAAAAAAAAAAAAAAAAAA670.1851493630309227No Hit
ACCATGAGCACTGTACGTACTCTGCGTTGATAC670.1851493630309227No Hit
GTACAGTGCTCAAAAAAAAAAAAAAAAAAAAAA630.17409566971564375No Hit
ACGCAGAGTGAATGGGCACTGTACGTAAAAAAA620.171332246386824No Hit
GCACTGTACGTACCAAAAAAAAAAAAAAAAAAA620.171332246386824No Hit
TATCTACGCAGAGTGAATGGGCACTGTACGTAC620.171332246386824No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT610.16856882305800425No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA590.16304197640036477No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA590.16304197640036477No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA590.16304197640036477No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA580.16027855307154504No Hit
AATGGGCACTGTACGTACAAAAAAAAAAAAAAA570.15751512974272527No Hit
CACTGTACGTACTCAAAAAAAAAAAAAAAAAAA570.15751512974272527No Hit
GAGTGAATGGGCACTGTACGTACTCAAAAAAAA570.15751512974272527No Hit
GCACTGTACGTAAAAAAAAAAAAAAAAAAAAAA550.1519882830850858No Hit
CACTGTACGTACAAAAAAAAAAAAAAAAAAAAA540.14922485975626607No Hit
GCACTGTACGTACTAAAAAAAAAAAAAAAAAAA530.14646143642744633No Hit
GAGCACTGTACGTACTCTGCGTTGATACCACTG520.14369801309862656No Hit
GTACGTACCAAAAAAAAAAAAAAAAAAAAAAAA510.14093458976980686No Hit
GTACGTACCTGAAAAAAAAAAAAAAAAAAAAAA510.14093458976980686No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA500.1381711664409871No Hit
CTCATGAGCACTGTACGTACTCTGCGTTGATAC490.13540774311216736No Hit
GTGAATGGGCACTGTACAAAAAAAAAAAAAAAA480.1326443197833476No Hit
AACGCAGAGTGAATGGGCACTGTACGTACTCAA480.1326443197833476No Hit
GCACTGTACAAAAAAAAAAAAAAAAAAAAAAAA460.12711747312570812No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA450.12435404979688838No Hit
GTACGTACCTGCAAAAAAAAAAAAAAAAAAAAA450.12435404979688838No Hit
GAGTGAATGGGCACTGTACGTAAAAAAAAAAAA440.12159062646806863No Hit
ATCAACGCAGAGTGAATGGGCACTGTACGTACT440.12159062646806863No Hit
GCATGAGCACTGTACGTACTCTGCGTTGATACC440.12159062646806863No Hit
CACATGAGCACTGTACGTACTCTGCGTTGATAC430.11882720313924891No Hit
GCACTGTACGTACCTAAAAAAAAAAAAAAAAAA420.11606377981042916No Hit
GAATGGGCACTGTACGTACTCAAAAAAAAAAAA410.11330035648160941No Hit
CAACGCAGAGTGAATGGGCACTGTACGTACAAA410.11330035648160941No Hit
TATCAACGCAGAGTGAATGGGCACTGTACGTAA400.11053693315278969No Hit
GTGAATGGGCATGAGCAAAAAAAAAAAAAAAAA390.10777350982396994No Hit
CTGTACGTACTCTGCAAAAAAAAAAAAAAAAAA380.10501008649515019No Hit
GTACGTACTAAAAAAAAAAAAAAAAAAAAAAAA380.10501008649515019No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA370.10224666316633044No Hit
ACGCTGAGTGAATGGGCACTGTACGTACAAAAA370.10224666316633044No Hit
GTCATGAGCACTGTACGTACTCTGCGTTGATAC370.10224666316633044No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAG200.00850442326.9986152
CTGAGTG200.00850442326.9986154
ACGCTGA257.6833763E-426.9986151
CAACTCA200.00850442326.9986151
GTATCAT405.8131627E-726.9986151
TCTACGC200.00850442326.9986153
TACGCAG200.00850442326.9986155
TATCATC405.8131627E-726.9986152
GCTGAGT200.00850442326.9986153
CTACGCA200.00850442326.9986154
TGAGTGA200.00850442326.9986155
TCGCAGA451.6237682E-623.9987687
ATCATCG451.6237682E-623.9987683
ATCGCAG451.6237682E-623.9987686
TCATCGC451.6237682E-623.9987684
TGGGGAC300.002223377622.4988466
GATACCA1600.021.966827
TGATACC1600.021.93637526
ACTACTC504.0558734E-621.5988923
CATCGCA504.0558734E-621.5988925
Produced by FastQC (version 0.11.2)