FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_52.3520000005ff9d.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_52.3520000005ff9d.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23526
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA4551.9340304344129897No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA1060.45056533197313614No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA990.42081101759755163No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA860.36555300518575196No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA710.3017937600952138No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA610.25928759670152174No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA570.24228513134404486No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA490.20828020062909122No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA480.204029584289722No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA460.1955283516109836No Hit
GTGAATGGGTATCGAAAAAAAAAAAAAAAAAAA400.17002465357476834No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA390.16577403723539913No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA390.16577403723539913No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA380.16152342089602992No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA380.16152342089602992No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA360.1530221882172915No Hit
CCCCAGGTCAGGCGGGATTACCCGCTGAGTTTA340.1445209555385531No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA330.14027033919918389No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC310.13176910652044546No Hit
GAGTGAATGGGTATCGACGAAAAAAAAAAAAAA300.12751849018107625No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA290.12326787384170704No Hit
GAGTGAATGGGTATCGACGTCAAAAAAAAAAAA290.12326787384170704No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA290.12326787384170704No Hit
GTGTATCGACGTCTACGTACTCTGCGTTGATAC290.12326787384170704No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA270.11476664116296864No Hit
GTTTAAGCATATCAAAAAAAAAAAAAAAAAAAA250.10626540848423022No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA240.102014792144861No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTCA240.102014792144861No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATACCA701.6711056E-719.28571527
TGATACC753.453315E-718.00000226
TTGATAC954.074678E-614.21052625
TCAACGC1203.2505377E-612.3753
TATCAAC1203.2505377E-612.3751
ATCAACG1203.2505377E-612.3752
TCTGCGT3350.011.68656827
GTTGATA1051.6649356E-411.57142824
CAACGCA1307.948103E-611.4230784
CGTTGAT1102.5655897E-411.04545523
CGCAGAG2653.6379788E-1210.69811252
ACGCAGA2552.3646862E-1110.5882351
AGTATCG900.00835454410.58
AACGCAG1555.5573764E-59.5806455
CTCTGCG4100.09.54878126
GCAGAGT2552.9467628E-99.5294113
TGTATCG1150.0046649449.3913058
CAGAGTG2502.2349923E-89.1799994
AGAGTGA2502.2349923E-89.1799995
GCGTTGA1350.00168821839.022
Produced by FastQC (version 0.11.2)