FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_51.3520000005ff73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_51.3520000005ff73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22563
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA5892.610468466072774No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA1580.700261490050082No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC1350.5983246908655764No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA770.3412666755307362No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA750.33240260603643135No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT700.3102424323006692No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA670.29694632805921195No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA590.26149005008199266No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA470.20830563311616362No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA470.20830563311616362No Hit
GTGAATGGGACTCTGTTCAAAAAAAAAAAAAAA400.1772813898860967No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA380.16841732039179189No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA320.14182511190887737No Hit
GTGAATGGGACTCTGTTCCAAAAAAAAAAAAAA300.13296104241457252No Hit
CCCCCACTCTGTTCCTCACGTACTCTGCGTTGA290.12852900766742012No Hit
GTTCCTCACGTACTCAAAAAAAAAAAAAAAAAA280.1240969729202677No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT260.11523290342596287No Hit
GAGTACGTGAGGAAAAAAAAAAAAAAAAAAAAA250.11080086867881045No Hit
GTGAATGGGGACTCTGTAAAAAAAAAAAAAAAA240.10636883393165802No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATACC1151.8189894E-1218.78415526
GATACCA1201.8189894E-1218.0014827
TATCAAC1400.017.3200841
GTTGATA1351.4551915E-1116.00131824
TTGATAC1351.4551915E-1116.00131825
TCAACGC1503.6379788E-1215.3012583
CGTTGAT1454.3655746E-1114.89777823
GCGTTGA3200.014.3449327
ATCAACG1551.2551027E-1013.9366312
GAAGCAG600.007249182613.5011119
GGAAGCA600.007249182613.5011118
GCACTCT1101.8414761E-512.2737387
CAACGCA1902.3646862E-1012.0799424
AACGCAG1955.7862053E-810.385475
TGCGTTG4600.09.97908226
AATGGGA1659.973386E-69.818994
TCTGCGT8050.09.559792527
GCAGAGT2502.2333006E-78.6407118
CTGCGTT5200.08.56801325
CACTCTG4650.08.4200488
Produced by FastQC (version 0.11.2)