FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_5.3520000005f9c9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_5.3520000005f9c9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGTAGCTCACGTACTCTGCGTTGATACCACTG80.7407407407407408No Hit
GTGTAGACGTAGCTCACGTACTCTGCGTTGATA70.6481481481481481No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA60.5555555555555556No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA60.5555555555555556No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.5555555555555556No Hit
GAGTGAATGGGGTAGACGTAAAAAAAAAAAAAA50.4629629629629629No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA50.4629629629629629No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA50.4629629629629629No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT40.3703703703703704No Hit
TATCAACGCAGAGTGAATGGGTAGACGTAGAAA40.3703703703703704No Hit
CATAGACGTAGCTCACGTACTCTGCGTTGATAC40.3703703703703704No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA40.3703703703703704No Hit
CCTAGACGTAGCTCACGTACTCTGCGTTGATAC40.3703703703703704No Hit
CCGTTAGACGTAGCTCACGTACTCTGCGTTGAT40.3703703703703704No Hit
CCCCTAGACGTAGCTCACGTACTCTGCGTTGAT40.3703703703703704No Hit
ACGCAGAGTGAATGGGTAGACGTAGAAAAAAAA30.2777777777777778No Hit
GTGAATGGGTAGACGTAGAAAAAAAAAAAAAAA30.2777777777777778No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA30.2777777777777778No Hit
GTACGTGAGCTACGTCTAAAAAAAAAAAAAAAA30.2777777777777778No Hit
CTTAGACGTAGCTCACGTACTCTGCGTTGATAC30.2777777777777778No Hit
GAGTGAATGGGTAGACGTAAAAAAAAAAAAAAA30.2777777777777778No Hit
CCCATAGACGTAGCTCACGTACTCTGCGTTGAT30.2777777777777778No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
GTGAATGGGTAGACGTAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
GGCTAGACGTAAAAAAAAAAAAAAAAAAAAAAA30.2777777777777778No Hit
ACCTAGACGTAGCTCACGTACTCTGCGTTGATA30.2777777777777778No Hit
GTAGACGTAGCTCACGTACTCTGCGTTGATACC30.2777777777777778No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC30.2777777777777778No Hit
GTCTAGACGTAGCTCACGTACTCTGCGTTGATA30.2777777777777778No Hit
GACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CCCTATAGACGTAGCTCACGTACTCTGCGTTGA20.1851851851851852No Hit
CCCTTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
GGATAGACGTAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CCCGTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
AGCTACGTCTATCAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGTAGACGTAGAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGGAGCTACGTCTA20.1851851851851852No Hit
TACTAGACGTAGCTCACGTACTCTGCAAAAAAA20.1851851851851852No Hit
CTTTAGACGTAGCTCACGTACTCTGCGTTGATA20.1851851851851852No Hit
CTAGACGTAGCTCACGTACTCTGCGTTGATACC20.1851851851851852No Hit
CAACGCAGAGTGAATGGGTAGACGTAGCTCACA20.1851851851851852No Hit
GTCTTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
GTCATAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA20.1851851851851852No Hit
GACCATAGACGTAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CTGTTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
ACCATAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
GATAGACGTAGCTCAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CTCTAGACGTAGCTCACGTACTCTGCGTTGATA20.1851851851851852No Hit
ACGTAGCTCACGTACTCAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGATAGACGTAGAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGGTGAGCTACGTCTAAAAAAAAAAA20.1851851851851852No Hit
GTATAGACGTAGCTCACGTACTCTGCGTTGATA20.1851851851851852No Hit
ACAGTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
AGTAGACGTAGCTCACGTACTCTGCGTTGATAC20.1851851851851852No Hit
CAACGCAGAGTGAATGGGAAGCAGTGGTATCAA20.1851851851851852No Hit
GTGAATGGGTAGACGTAGCTCAAAAAAAAAAAA20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGCTAGAAAAAAAA20.1851851851851852No Hit
GTTCAGACGTGTGCTCTTCAAAAAAAAAAAAAA20.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGATAGACGTAGCTC20.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGCCACTAGACGAA20.1851851851851852No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
AAACAAAAAGAACGAAAAAGCAAAAGGAGAAAC20.1851851851851852No Hit
GTGAATGGGATACTAGACGTAAAAAAAAAAAAA20.1851851851851852No Hit
ATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTGAATGGGATAGACGTAAAAAAAAAAAAAAAA20.1851851851851852No Hit
CCCTAGACGTAGCTCACGTACTCTGCGTTGATA20.1851851851851852No Hit
CCATAGAGTAGACGTAGCTCACGTACTCTGCGT20.1851851851851852No Hit
GCTCACGTACTCTGCGTAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GAATAGACGTAGCTCACGTACTCTGCGTTGATA20.1851851851851852No Hit
AAAAAGTAGTTCCTAGACGTAGCTCACGTACTC20.1851851851851852No Hit
CCATTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
GTGCTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit
AGACGTAGCTCACGTACTCTGCGTTGATACCAC20.1851851851851852No Hit
GTATCAACGCAAAAAAAAAAAAAAAAAAAAAAA20.1851851851851852No Hit
GTATTAGACGTAGCTCACGTACTCTGCGTTGAT20.1851851851851852No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)