FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_49.3520000005ff30.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_49.3520000005ff30.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences64273
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CACACGTACTCAAAAAAAAAAAAAAAAAAAAAA15382.3929177103916106No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA12741.9821698069173679No Hit
ACGCAGAGTGAATGGGCAGACACAAAAAAAAAA9931.5449722278406175No Hit
GTGAATGGGCAGACACAAAAAAAAAAAAAAAAA9421.4656232010330932No Hit
TATCAACGCAGAGTGAATGGGCAGACACAAAAA7771.2089057613616914No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGTA7731.2026823082787483No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCAA5710.8883979275901235No Hit
AACGCAGAGTGAATGGGCAGACACAAAAAAAAA5390.8386103029265788No Hit
GTGAATGGGCAGACACACGTACTCAAAAAAAAA5370.8354985763851073No Hit
GAGTGAATGGGCAGACACAAAAAAAAAAAAAAA5040.7841550884508269No Hit
ACGCAGAGTGAATGGGCAGACAAAAAAAAAAAA4690.7296998739750751No Hit
GTGAATGGGCAGACAAAAAAAAAAAAAAAAAAA4480.6970267452896239No Hit
GACACACGTACTCAAAAAAAAAAAAAAAAAAAA4350.676800522770059No Hit
CACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4270.6643536166041728No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA4170.6487949838968152No Hit
AACGCAGAGTGAATGGGCAGACACACGTACTCA3670.5710018203600268No Hit
GTATCAACGCAGAGTGAATGGGCAGACACACGT3630.5647783672770837No Hit
TATCAACGCAGAGTGAATGGGCAGACAAAAAAA3310.5149907426135392No Hit
GTATCAACGCAGAGTGAATGGGCAGACACAAAA3180.4947645200939741No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3100.482317613928088No Hit
GAGTGAATGGGCAGACACACGTACTCAAAAAAA3030.47142657103293767No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGT2910.4527562117841084No Hit
GACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA2850.44342103215969386No Hit
GAGCAGACACACGTACTCTGCGTTGATACCACT2810.4371975790767507No Hit
GTATCATCGCAGAGTGAATGGGCAGACACAAAA2720.4231948096401288No Hit
ATCAACGCAGAGTGAATGGGCAGACACAAAAAA2700.4200830830986573No Hit
TATCTACGCAGAGTGAATGGGCAGACACAAAAA2590.4029685871205639No Hit
ATCAACGCAGAGTGAATGGGCAGACACACGTAC2370.36873959516437693No Hit
AACGCAGAGTGAATGGGCAGACAAAAAAAAAAA2360.3671837318936412No Hit
CAACTCAGAGTGAATGGGCAGACACAAAAAAAA2360.3671837318936412No Hit
CACGTAAAAAAAAAAAAAAAAAAAAAAAAAAAA2350.3656278686229054No Hit
GAGTGAATGGGCAGACAAAAAAAAAAAAAAAAA2280.35473682572775506No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGTA2260.35162509918628354No Hit
CACACGTACTCTAAAAAAAAAAAAAAAAAAAAA2160.3360664664789258No Hit
CAACGCAGAGTGAATGGGCAGACACAAAAAAAA2070.3220636970423039No Hit
ACGCAGAGTGAATGGGCAGACACACAAAAAAAA1930.30028161125200314No Hit
GTGAATGGGCAGACACACAAAAAAAAAAAAAAA1930.30028161125200314No Hit
CACGTACAAAAAAAAAAAAAAAAAAAAAAAAAA1860.28939056835685284No Hit
GTGAATGGGCAGACACACGAAAAAAAAAAAAAA1820.28316711527390975No Hit
ACGCAGAGTGAATGGGCAGACACACGAAAAAAA1770.2753877989202309No Hit
CAGACACACGTACTCAAAAAAAAAAAAAAAAAA1670.2598291662128732No Hit
CACGTACTCTAAAAAAAAAAAAAAAAAAAAAAA1660.25827330294213746No Hit
CAACTCAGAGTGAATGGGCAGACAAAAAAAAAA1470.22871190079815787No Hit
AACGTAGAGTGAATGGGCAGACACAAAAAAAAA1460.22715603752742208No Hit
TATCAACGCAGAGTGAATGGGCAGACACACGAA1450.22560017425668633No Hit
CAACGCAGAGTGAATGGGCAGACACACGTACTC1410.21937672117374324No Hit
TATCAACGCAGAGTGAATGGGCAGACACACAAA1350.21004154154932864No Hit
ATCAACGCAGAGTGAATGGGCAGACAAAAAAAA1340.20848567827859288No Hit
GACACACAAAAAAAAAAAAAAAAAAAAAAAAAA1310.20381808846638558No Hit
TATCTACGCAGAGTGAATGGGCAGACAAAAAAA1290.20070636192491406No Hit
ACGCAGAGTGAATGGGCAGACACACGTAAAAAA1270.1975946353834425No Hit
GACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1270.1975946353834425No Hit
CACACAAAAAAAAAAAAAAAAAAAAAAAAAAAA1260.19603877211270673No Hit
GTATCAACGCAGAGTGAATGGGCAGACAAAAAA1240.19292704557123522No Hit
ACGCTGAGTGAATGGGCAGACACAAAAAAAAAA1220.18981531902976367No Hit
AACGCAGAGTGAATGGGCAGACACACAAAAAAA1140.17736841286387753No Hit
AACGCAGAGTGAATGGGCAGACACACGAAAAAA1090.16958909651019868No Hit
GTGAATGGGCAGACACACGTAAAAAAAAAAAAA1070.16647736996872714No Hit
GTATCATCGCAGAGTGAATGGGCAGACAAAAAA1060.16492150669799138No Hit
CAACTCAGAGTGAATGGGCAGACACACGTACTC1060.16492150669799138No Hit
GAGTGAATGGGCAGACACACAAAAAAAAAAAAA1040.16180978015651987No Hit
CACGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1030.16025391688578408No Hit
CACACGTACTCTGAAAAAAAAAAAAAAAAAAAA1020.15869805361504832No Hit
CACACGAAAAAAAAAAAAAAAAAAAAAAAAAAA1000.15558632707357678No Hit
GGCAGACACACGTACTCAAAAAAAAAAAAAAAA980.15247460053210524No Hit
TCAACGCAGAGTGAATGGGCAGACACAAAAAAA980.15247460053210524No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.14625114744916218No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACACG910.14158355763695488No Hit
AACGTAGAGTGAATGGGCAGACACACGTACTCA890.13847183109548333No Hit
GGCAGACACAAAAAAAAAAAAAAAAAAAAAAAA850.13224837801254025No Hit
CACACGTAAAAAAAAAAAAAAAAAAAAAAAAAA850.13224837801254025No Hit
TCAACGCAGAGTGAATGGGCAGACACACGTACT830.12913665147106873No Hit
CTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA830.12913665147106873No Hit
GAGTAAATGGGCAGACACAAAAAAAAAAAAAAA830.12913665147106873No Hit
GAGTGAATGGGCAGACACACGAAAAAAAAAAAA820.12758078820033297No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACGA810.1260249249295972No Hit
GGTATCAACGCAGAGTGAATGGGCAGACACAAA800.12446906165886143No Hit
CAACGCAGAGTGAATGGGCAGACAAAAAAAAAA790.12291319838812564No Hit
ACGCTGAGTGAATGGGCAGACACACGTACTCAA750.11668974530518257No Hit
ATCAACGCAGAGTGAATGGGCAGACACACAAAA730.11357801876371104No Hit
GTATCATCGCAGAGTGAATGGGCAGACACACAA720.11202215549297528No Hit
ACGCAGAGTGAATGGGCAGACACACGTACTCTG710.11046629222223953No Hit
AACGCAGAGTGAATGGGCAGACACACGTAAAAA690.10735456568076798No Hit
CGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.10735456568076798No Hit
CCCATGAGCAGACACACGTACTCTGCGTTGATA680.1057987024100322No Hit
GAATGGGCAGACACAAAAAAAAAAAAAAAAAAA680.1057987024100322No Hit
TATCTACGCAGAGTGAATGGGCAGACACACGAA650.10113111259782491No Hit
ACGCAGAGTGAATGGGCAGACACACGTACAAAA650.10113111259782491No Hit
GCAGACACACGTACTCAAAAAAAAAAAAAAAAA650.10113111259782491No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTCA257.7131705E-427.0202641
TTCAACG307.056839E-526.9992224
TTTCAAC307.056839E-526.9992223
GTTTCAA257.7484275E-426.9992222
GTATCAT1150.025.845471
CAACTCA1050.025.7335851
TATCTAC1200.024.7685761
TATCATC1200.024.7492872
GGTATCA501.5055593E-724.3182371
ATCTACG1250.023.7593142
TCTACGC1250.023.7593143
GTATGGG352.0104111E-423.1421893
TGTATGG352.0104111E-423.1421892
TACGCAG1300.022.8454955
CTACGCA1300.022.8454954
ACGCTGA300.00223180622.5168881
CGCTGAG300.002241952822.4993522
TATGGGC300.002241952822.4993524
ATCATCG1300.021.8070623
TCATCGC1300.021.8070624
Produced by FastQC (version 0.11.2)