FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_48.3520000005ff16.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_48.3520000005ff16.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCCACGCTTAACGTACTCTGCGTTGATACCA633.088235294117647No Hit
GTGAATGGGTTCCACGCAAAAAAAAAAAAAAAA160.7843137254901961No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA110.5392156862745098No Hit
GTGAATGGGTTCCACAAAAAAAAAAAAAAAAAA100.49019607843137253No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.39215686274509803No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA70.3431372549019608No Hit
TTCCACGCTTAACGTACTCTGCGTTGATACCAC70.3431372549019608No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA50.24509803921568626No Hit
ACGCAGAGTGAATGGGTTCCACAAAAAAAAAAA50.24509803921568626No Hit
GTGAATGGGGTTCCACGCAAAAAAAAAAAAAAA40.19607843137254902No Hit
AACGCAGAGTGAATGGGTTCCACAAAAAAAAAA40.19607843137254902No Hit
ACGCAGAGTGAATGGGGTTCCACAAAAAAAAAA40.19607843137254902No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA40.19607843137254902No Hit
GTTCCACGCAAAAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
CTGTTCCACGCTTAACGTACTCTGCGTTGATAC30.14705882352941177No Hit
GTGAATGGGAGTTCCACAAAAAAAAAAAAAAAA30.14705882352941177No Hit
AACGCAGAGTGAATGGGTTCCACGCAAAAAAAA30.14705882352941177No Hit
GAGTGAATGGGTTCCACAAAAAAAAAAAAAAAA30.14705882352941177No Hit
CGCTTAACGTACTCAAAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
GTATCAACGCAGAGTGAATGGGGTTCCACAAAA30.14705882352941177No Hit
CAACGCAGAGTGAATGGGTTCCACAAAAAAAAA30.14705882352941177No Hit
CGTTAAGCGTGGAAAAAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
CTCCCGTTCCACGCTTAACGTACTCTGCGTTGA30.14705882352941177No Hit
GTACGTTAAGCGTGGAAAAAAAAAAAAAAAAAA30.14705882352941177No Hit
GAGTGAATGGGTTCCACGCAAAAAAAAAAAAAA30.14705882352941177No Hit
CCCCACGTTCCACGCTTAACGTACTCTGCGTTG30.14705882352941177No Hit
ACGCAGAGTGAATGGGAGTTCCACAAAAAAAAA30.14705882352941177No Hit
AACGCAGAGTGAATGGGTTCCAAAAAAAAAAAA30.14705882352941177No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)