Basic Statistics
Measure | Value |
---|---|
Filename | HTC23BCXX_l02n02_kb_plate7_46.3520000005feee.fastq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 8941 |
Sequences flagged as poor quality | 0 |
Sequence length | 33 |
%GC | 43 |
Per base sequence quality
Per tile sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC | 61 | 0.68225030757186 | No Hit |
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 36 | 0.4026395257801141 | No Hit |
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA | 33 | 0.3690862319651046 | No Hit |
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA | 29 | 0.3243485068784252 | No Hit |
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC | 27 | 0.30197964433508556 | No Hit |
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA | 26 | 0.2907952130634157 | No Hit |
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA | 14 | 0.1565820378033777 | No Hit |
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA | 12 | 0.13421317526003804 | No Hit |
ACTCCTTGCAGTAACGTACTCTGCGTTGATACC | 11 | 0.12302874398836819 | No Hit |
CCCCCCCTCCTTGCAGTAACGTACTCTGCGTTG | 11 | 0.12302874398836819 | No Hit |
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC | 11 | 0.12302874398836819 | No Hit |
TCTCCTTGCAGTAACGTACTCTGCGTTGATACC | 9 | 0.10065988144502852 | No Hit |
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 9 | 0.10065988144502852 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
TGCGTTG | 75 | 6.070708E-6 | 16.199999 | 27 |
TCTGCGT | 285 | 0.0 | 13.736842 | 27 |
TCTCCTT | 75 | 0.001963044 | 12.599999 | 8 |
CTGCGTT | 140 | 7.58082E-8 | 12.535714 | 27 |
CTCTGCG | 385 | 0.0 | 10.168832 | 26 |
ACTCTGC | 410 | 0.0 | 9.219512 | 25 |
AACGTAC | 470 | 0.0 | 8.329787 | 20 |
GTACTCT | 470 | 0.0 | 8.329787 | 23 |
CGTACTC | 470 | 0.0 | 8.329787 | 22 |
TAACGTA | 475 | 0.0 | 8.242105 | 19 |
ACGTACT | 475 | 0.0 | 8.242105 | 21 |
TACTCTG | 465 | 0.0 | 8.129032 | 24 |
GTAACGT | 485 | 0.0 | 8.072165 | 18 |
AGTAACG | 490 | 0.0 | 7.989796 | 17 |
ACTCCTT | 155 | 0.005195032 | 7.8387103 | 8 |
CAGTAAC | 505 | 0.0 | 7.7524753 | 16 |
GCAGTAA | 505 | 0.0 | 7.7524753 | 15 |
TGCAGTA | 505 | 0.0 | 7.7524753 | 14 |
TTGCAGT | 510 | 0.0 | 7.6764708 | 13 |
CTCCTTG | 510 | 0.0 | 7.6764708 | 9 |