FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_46.3520000005feee.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_46.3520000005feee.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8941
Sequences flagged as poor quality0
Sequence length33
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTTGCAGTAACGTACTCTGCGTTGATACCAC610.68225030757186No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA360.4026395257801141No Hit
GTACGTTACTGCAAAAAAAAAAAAAAAAAAAAA330.3690862319651046No Hit
CTCCTTGCAGTAACGTACTCTGCGTTGATACCA290.3243485068784252No Hit
CCTCCTTGCAGTAACGTACTCTGCGTTGATACC270.30197964433508556No Hit
GAGTACGTTACTGCAAAAAAAAAAAAAAAAAAA260.2907952130634157No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA140.1565820378033777No Hit
GTGAATGGGGTACGTTACTGCAAAAAAAAAAAA120.13421317526003804No Hit
ACTCCTTGCAGTAACGTACTCTGCGTTGATACC110.12302874398836819No Hit
CCCCCCCTCCTTGCAGTAACGTACTCTGCGTTG110.12302874398836819No Hit
GCTCCTTGCAGTAACGTACTCTGCGTTGATACC110.12302874398836819No Hit
TCTCCTTGCAGTAACGTACTCTGCGTTGATACC90.10065988144502852No Hit
CTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.10065988144502852No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGTTG756.070708E-616.19999927
TCTGCGT2850.013.73684227
TCTCCTT750.00196304412.5999998
CTGCGTT1407.58082E-812.53571427
CTCTGCG3850.010.16883226
ACTCTGC4100.09.21951225
AACGTAC4700.08.32978720
GTACTCT4700.08.32978723
CGTACTC4700.08.32978722
TAACGTA4750.08.24210519
ACGTACT4750.08.24210521
TACTCTG4650.08.12903224
GTAACGT4850.08.07216518
AGTAACG4900.07.98979617
ACTCCTT1550.0051950327.83871038
CAGTAAC5050.07.752475316
GCAGTAA5050.07.752475315
TGCAGTA5050.07.752475314
TTGCAGT5100.07.676470813
CTCCTTG5100.07.67647089