FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_44.3520000005feab.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_44.3520000005feab.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3420
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGAATGGGCAGAGTGGCAAAAAAAAAAAAAAA200.5847953216374269No Hit
ACGCAGAGTGAATGGGCAGAGTGGCAAAAAAAA180.5263157894736842No Hit
GGCTAAACGTACTCAAAAAAAAAAAAAAAAAAA120.3508771929824561No Hit
GAGTGGAAAAAAAAAAAAAAAAAAAAAAAAAAA120.3508771929824561No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.32163742690058483No Hit
GAGTGAATGGGCAGAGTGGCAAAAAAAAAAAAA100.29239766081871343No Hit
GTGGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.29239766081871343No Hit
GTGAATGGGGCAGAGTGGCAAAAAAAAAAAAAA90.2631578947368421No Hit
GTATCAACGCAGAGTGAATGGGCAGAGTGGCAA90.2631578947368421No Hit
GTGAATGGGCAGAGTGAAAAAAAAAAAAAAAAA90.2631578947368421No Hit
TATCAACGCAGAGTGAATGGGCAGAGTGGCAAA80.23391812865497078No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACTC70.20467836257309943No Hit
AACGCAGAGTGAATGGGCAGAGTGGCAAAAAAA70.20467836257309943No Hit
GTATCAACGCAGAGTGAATGGGCTAAACGTACT70.20467836257309943No Hit
GGGCAGAGTGGCAAAAAAAAAAAAAAAAAAAAA70.20467836257309943No Hit
AACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA70.20467836257309943No Hit
CAACGCAGAGTGAATGGGCAGAGTGGCAAAAAA60.17543859649122806No Hit
GAATGGGCAGAGTGGCAAAAAAAAAAAAAAAAA60.17543859649122806No Hit
GAGTGAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.17543859649122806No Hit
GTGAATGGGCAGAGTGGAAAAAAAAAAAAAAAA60.17543859649122806No Hit
GTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.17543859649122806No Hit
AACGCAGAGTGAATGGGCAGAGTGGAAAAAAAA60.17543859649122806No Hit
CCCCAGAGTGGCTAAACGTACTCTGCGTTGATA50.14619883040935672No Hit
GTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.14619883040935672No Hit
GTCCAGAGTGGCTAAACGTACTCTGCGTTGATA50.14619883040935672No Hit
GAGTGGCAAAAAAAAAAAAAAAAAAAAAAAAAA50.14619883040935672No Hit
ACGCAGAGTGAATGGGACAGAGTGGAAAAAAAA50.14619883040935672No Hit
ATCAACGCAGAGTGAATGGGCAGAGTGGCAAAA50.14619883040935672No Hit
CCACCGACCAGAGTGGCTAAACGTACTCTGCGT40.11695906432748539No Hit
CCCCCCAGAGTGGCTAAACGTACTCTGCGTTGA40.11695906432748539No Hit
GAATGGGACAGAGTGGCAAAAAAAAAAAAAAAA40.11695906432748539No Hit
GGACAGAGTGGAAAAAAAAAAAAAAAAAAAAAA40.11695906432748539No Hit
CCCCCAGAGTGGCTAAACGTACTCTGCGTTGAT40.11695906432748539No Hit
GTGAATGGGCTAAACGTACAAAAAAAAAAAAAA40.11695906432748539No Hit
CCTCCAGAGTGGCTAAACGTACTCTGCGTTGAT40.11695906432748539No Hit
CCGCAGAGTGGCTAAACGTACTCTGCGTTGATA40.11695906432748539No Hit
TATCAACGCAGAGTGAATGGGCTAAACGTACCT40.11695906432748539No Hit
GAGTGAATGGGACAGAGTGGCAAAAAAAAAAAA40.11695906432748539No Hit
GCCCAGAGTGGCTAAACGTACTCTGCGTTGATA40.11695906432748539No Hit
CACTCAGAGTGGCTAAACGTACTCTGCGTTGAT40.11695906432748539No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT801.4570105E-818.562527
CTCTGCG1159.302985E-712.91304426
CCAGAGT904.010586E-412.08
TACTCTG1353.6351958E-712.024
ACTCTGC1303.7203881E-611.42307825
GTACTCT1458.7009903E-711.17241423
ACGTACT1501.3145054E-610.79999921
CGTACTC1501.3145054E-610.79999922
AACGTAC1551.9573781E-610.45161220
TAAACGT1551.9573781E-610.45161218
AGTGGCT1551.9573781E-610.45161212
GTGGCTA1551.9573781E-610.45161213
AAACGTA1551.9573781E-610.45161219
TGGCTAA1551.9573781E-610.45161214
GCTAAAC1602.875444E-610.12516
CTAAACG1602.875444E-610.12517
GGCTAAA1654.170759E-69.81818115
GAGTGGC1751.01993675E-48.48571511
AGAGTGG1953.334502E-47.61538410
CAGAGTG2850.0025782735.68421039
Produced by FastQC (version 0.11.2)