FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_42.3520000005fe5b.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_42.3520000005fe5b.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10381
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AACGTACTCTGCGTAAAAAAAAAAAAAAAAAAA290.2793565167132261No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA180.17339370002889895No Hit
GTGAATGGGCAGTCTCAAAAAAAAAAAAAAAAA140.13486176668914363No Hit
AACGTACTCTGCGTTGATACAAAAAAAAAAAAA130.12522878335420481No Hit
AACGTACTCTGCGTTGATACCAAAAAAAAAAAA110.10596281668432714No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT2200.015.34090927
CCAGTCT1252.942088E-712.969
TCAGTCT1000.001344626310.7999998
CTCTGCG3350.09.67164126
GGAAACG3701.8189894E-128.75675717
GAAACGT3751.8189894E-128.63999918
ACTCTGC3751.8189894E-128.63999925
TGGAAAC3601.0913936E-118.62516
AAACGTA3803.6379788E-128.52631619
GTCTCTG3752.7284841E-118.2811
CTCTGGA3803.6379788E-118.17105313
TCTCTGG3803.6379788E-118.17105312
GTACTCT4001.0913936E-118.09999923
TACTCTG4001.0913936E-118.09999924
TCTGGAA3854.9112714E-118.06493514
CAGTCTC4051.4551915E-118.09
CTGGAAA3906.366463E-117.96153915
ACGTACT4102.0008883E-117.902438621
CGTACTC4102.0008883E-117.902438622
AGTCTCT3958.367351E-117.860759310
Produced by FastQC (version 0.11.2)