FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_41.3520000005fe31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_41.3520000005fe31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3421
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTATCGGTTGAACGTACTCTGCGTTGATACCA621.8123355743934522No Hit
GCCTATCGGTTGAACGTACTCTGCGTTGATACC130.38000584624378836No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA130.38000584624378836No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.3215434083601286No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA90.2630809704764689No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA90.2630809704764689No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA90.2630809704764689No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA90.2630809704764689No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA80.233849751534639No Hit
GTGAATGGGCCTATCGGAAAAAAAAAAAAAAAA80.233849751534639No Hit
CCCTATCGGTTGAACGTACTCTGCGTTGATACC80.233849751534639No Hit
ACGCAGAGTGAATGGGCCTATCGGTAAAAAAAA80.233849751534639No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA80.233849751534639No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT70.20461853259280913No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA70.20461853259280913No Hit
GAGTACGTTCAAAAAAAAAAAAAAAAAAAAAAA70.20461853259280913No Hit
ACGCAGAGTGAATGGGCCTATCGGAAAAAAAAA60.17538731365097923No Hit
ATCCTATCGGTTGAACGTACTCTGCGTTGATAC60.17538731365097923No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT50.14615609470914936No Hit
GCCTATCGGTAAAAAAAAAAAAAAAAAAAAAAA50.14615609470914936No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA50.14615609470914936No Hit
AACGCAGAGTGAATGGGCCTATCGGAAAAAAAA50.14615609470914936No Hit
CCCCTATCGGTTGAACGTACTCTGCGTTGATAC50.14615609470914936No Hit
GTCCTATCGGTTGAACGTACTCTGCGTTGATAC50.14615609470914936No Hit
ACCCTATCGGTTGAACGTACTCTGCGTTGATAC40.1169248757673195No Hit
CTCCTATCGGTTGAACGTACTCTGCGTTGATAC40.1169248757673195No Hit
TATCAACGCAGAGTGAATGGGCCTATCGGAAAA40.1169248757673195No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT851.1768861E-919.05882527
CTCTGCG1155.0584276E-814.08695726
ACTCTGC1251.4138641E-712.95999925
TACTCTG1251.4138641E-712.95999924
CGTACTC1403.4318873E-812.53571422
AACGTAC1455.455513E-812.10344920
GTTGAAC1455.455513E-812.10344916
GAACGTA1455.455513E-812.10344919
ACGTACT1455.455513E-812.10344921
TGAACGT1455.455513E-812.10344918
GTACTCT1353.6351958E-712.023
GGTTGAA1508.529241E-811.715
TCGGTTG1508.529241E-811.713
ATCGGTT1508.529241E-811.712
CGGTTGA1508.529241E-811.714
TTGAACG1551.3128374E-711.3225817
TATCGGT1501.3145054E-610.79999911
CTATCGG1801.1878892E-59.010
CCTATCG1902.267506E-58.5263169
Produced by FastQC (version 0.11.2)