FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_40.3520000005fe17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_40.3520000005fe17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5941
Sequences flagged as poor quality0
Sequence length33
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCAAGCTCAACACGTACTCTGCGTTGATACCA871.4643999326712673No Hit
CGTCAAGCTCAACACGTACTCTGCGTTGATACC270.45446894462211745No Hit
GTGAATGGGTCAAGCTCAAAAAAAAAAAAAAAA240.40397239521965994No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.33664366268304996No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA180.30297929641474497No Hit
GCTCAACACGTACTCAAAAAAAAAAAAAAAAAA130.2188183807439825No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA110.1851540144756775No Hit
CCGTCAAGCTCAACACGTACTCTGCGTTGATAC110.1851540144756775No Hit
GGTCAAGCTCAACACGTACTCTGCGTTGATACC100.16832183134152498No Hit
AACGCAGAGTGAATGGGTCAAGCTCAAAAAAAA100.16832183134152498No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA90.15148964820737248No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT70.1178252819390675No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA70.1178252819390675No Hit
ACGCAGAGTGAATGGGTCAAGCTCAAAAAAAAA70.1178252819390675No Hit
GTGAATGGGTCAAGCAAAAAAAAAAAAAAAAAA70.1178252819390675No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA70.1178252819390675No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA60.10099309880491499No Hit
ACGCAGAGTGAATGGGTCAAGCTAAAAAAAAAA60.10099309880491499No Hit
GTCAAGCTCAAAAAAAAAAAAAAAAAAAAAAAA60.10099309880491499No Hit
GCTCAACACAAAAAAAAAAAAAAAAAAAAAAAA60.10099309880491499No Hit
GAGTGAATGGGTCAAGCTCAAAAAAAAAAAAAA60.10099309880491499No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA60.10099309880491499No Hit
GCTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.10099309880491499No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAATGG350.004919511319.2857152
Produced by FastQC (version 0.11.2)