FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_4.3520000005f9a0.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_4.3520000005f9a0.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCGACGTCTACGTACTCTGCGTTGATACCA693.194444444444444No Hit
GTGAATGGGTATCGACGTCAAAAAAAAAAAAAA200.9259259259259258No Hit
GTGAATGGGTATCGACGTAAAAAAAAAAAAAAA190.8796296296296297No Hit
GTGAATGGGTATCGACGAAAAAAAAAAAAAAAA190.8796296296296297No Hit
GTATCGACGTCAAAAAAAAAAAAAAAAAAAAAA130.6018518518518519No Hit
ACGCAGAGTGAATGGGTATCGACGTAAAAAAAA130.6018518518518519No Hit
GTGAATGGGGTATCGACGTCAAAAAAAAAAAAA120.5555555555555556No Hit
GTATCGACGTAAAAAAAAAAAAAAAAAAAAAAA110.5092592592592593No Hit
ACGCAGAGTGAATGGGTATCGACGAAAAAAAAA110.5092592592592593No Hit
AACGCAGAGTGAATGGGTATCGACGAAAAAAAA100.4629629629629629No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA80.3703703703703704No Hit
GTGAATGGGTATCGACAAAAAAAAAAAAAAAAA70.32407407407407407No Hit
TATCAACGCAGAGTGAATGGGTATCGACGAAAA70.32407407407407407No Hit
GTGAATGGGGTATCGACGAAAAAAAAAAAAAAA60.2777777777777778No Hit
AACGCAGAGTGAATGGGTATCGACGTAAAAAAA60.2777777777777778No Hit
GAGTGAATGGGTATCGACGTAAAAAAAAAAAAA50.23148148148148145No Hit
ACGCAGAGTGAATGGGTATCGACAAAAAAAAAA50.23148148148148145No Hit
GTGAATGGGTATCGACGTCTACAAAAAAAAAAA50.23148148148148145No Hit
CCCCCCGTATCGACGTCTACGTACTCTGCGTTG50.23148148148148145No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTAAAA50.23148148148148145No Hit
GGTATCGACGTCTACGTACTCTGCGTTGATACC40.1851851851851852No Hit
ACGCAGAGTGAATGGGTATCGACGTCAAAAAAA40.1851851851851852No Hit
ACCCAGTATCGACGTCTACGTACTCTGCGTTGA40.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCTA40.1851851851851852No Hit
GTGAATGGGAGTATCGACGTCAAAAAAAAAAAA40.1851851851851852No Hit
CCCCCGTATCGACGTCTACGTACTCTGCGTTGA40.1851851851851852No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGGTATCGACGTAAAAAAAAAAAAAA40.1851851851851852No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GTATCGACGAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
AACGCAGAGTGAATGGGTATCGACGTCAAAAAA40.1851851851851852No Hit
AACGCAGAGTGAATGGGGTATCGACAAAAAAAA40.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA40.1851851851851852No Hit
CGTATCGACGTCTACGTACTCTGCGTTGATACC40.1851851851851852No Hit
GTGAATGGGAGTATCGACGTAAAAAAAAAAAAA40.1851851851851852No Hit
ACGCAGAGTGAATGGGGTATCGACGTCAAAAAA40.1851851851851852No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTCTAC40.1851851851851852No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GTGAATGGGGTATCGACAAAAAAAAAAAAAAAA40.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTCAA40.1851851851851852No Hit
TCTACGTACTCAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT30.1388888888888889No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTCAAA30.1388888888888889No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA30.1388888888888889No Hit
ACGCAGAGTGAATGGGCCCCGTATCAAAAAAAA30.1388888888888889No Hit
ATCAACGCAGAGTGAATGGGTATCGACGAAAAA30.1388888888888889No Hit
GCGTATCGACGTCTACGTACTCTGCGTTGATAC30.1388888888888889No Hit
TATCTACGCAGAGTGAATGGGTATCGACGTAAA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGTATCGACGTAAA30.1388888888888889No Hit
GGTATCAACGCAGAGTGAATGGGTATCGACGTA30.1388888888888889No Hit
GTATCGACGTCTACGTACAAAAAAAAAAAAAAA30.1388888888888889No Hit
AACGCAGAGTGAATGGGGTATCGACGAAAAAAA30.1388888888888889No Hit
GGGTATCGACGAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGTAA30.1388888888888889No Hit
GAGTGAATGGGGTATCGACAAAAAAAAAAAAAA30.1388888888888889No Hit
ATCAACGCAGAGTGAATGGGTATCGACGTCTAA30.1388888888888889No Hit
CAACGCAGAGTGAATGGGTATCGACGTCAAAAA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGGTATCGACGAAA30.1388888888888889No Hit
GTATCAACGCAGAGTGAATGGGTATCGACGAAA30.1388888888888889No Hit
GGTATCGACGAAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT350.003850145319.3537427
GTGAATG600.004636282613.547621
TGAATGG600.004636282613.547622
TCGACGT1100.00154656389.85281512
GTATCGA1400.00121367148.5066449
TATCGAC1300.0062577398.33699610
ATCGACG1300.0062577398.33699611
Produced by FastQC (version 0.11.2)