FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_39.3520000005fdfc.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_39.3520000005fdfc.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100
Sequences flagged as poor quality0
Sequence length33
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA100.4761904761904762No Hit
ACGCAGAGTGAATGGGAGCTGACGAAAAAAAAA90.4285714285714286No Hit
AGCTGACGAAGTACGTACTCTGCGTTGATACCA90.4285714285714286No Hit
GTGAATGGGAGCTGACGAAAAAAAAAAAAAAAA90.4285714285714286No Hit
CTGACGAAGTACGTACTCTGCGTTGATACCACT90.4285714285714286No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA80.38095238095238093No Hit
GCTGACGAAGTACGTACTCTGCGTTGATACCAC80.38095238095238093No Hit
TATCAACGCAGAGTGAATGGGAGCTGACGAAAA80.38095238095238093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.38095238095238093No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
GTGAATGGGAGCTGACAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA70.33333333333333337No Hit
GCTAAAGGAAGGGAAATCAACCGAGACCCCGTT60.2857142857142857No Hit
GATTTGGGATTTTAAAAAAAAAAAAAAAAAAAA60.2857142857142857No Hit
TAGTAGCGGCGAGCGAGAGCGGATTTGGGATTT60.2857142857142857No Hit
GTGTAGATCTCGGTGGTCGCCGTATCATTAAAA60.2857142857142857Illumina Single End PCR Primer 1 (100% over 29bp)
CAGCTGACGAAGTACGTACTCTGCGTTGATACC60.2857142857142857No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA50.2380952380952381No Hit
GAGTACGTACTCTGCGTAAAAAAAAAAAAAAAA50.2380952380952381No Hit
GAGTGAATGGGGCTGACGAAAAAAAAAAAAAAA50.2380952380952381No Hit
AACGCAGAGTGAATGGGAGCTGACGAAAAAAAA50.2380952380952381No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA50.2380952380952381No Hit
GAGCTGACGAAGTACAAAAAAAAAAAAAAAAAA50.2380952380952381No Hit
CATCTAAGTAGCTAAAAAAAAAAAAAAAAAAAA50.2380952380952381No Hit
GGGAGATACCGTCTGTGATCCATGGATCTCCGA50.2380952380952381No Hit
AAGCTGACGAAGTACGTACTCTGCGTTGATACC40.19047619047619047No Hit
TGGCTAGTCTGCGCTCTTTGGACTTTGAAAACT40.19047619047619047No Hit
CGTTAGTAGCGGCGAGCGAGAGCGGATTTGGGA40.19047619047619047No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA40.19047619047619047No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
CCCCCCAGCTGACGAAGTACGTACTCTGCGTTG40.19047619047619047No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA40.19047619047619047No Hit
GTGAATGGGAAGCTGACGAAGTACAAAAAAAAA40.19047619047619047No Hit
GAGTGAATGGGCTGACGAAAAAAAAAAAAAAAA40.19047619047619047No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC40.19047619047619047No Hit
GAGCTGACGAAGTACGTACTCTGCGTTGATACC40.19047619047619047No Hit
CATCTAAGTAGCTAAAGGAAGGGAAATCAACCG30.14285714285714285No Hit
TCCAAGCTCCGTGGCTAGTCTGCGCTCTTTGGA30.14285714285714285No Hit
GTGAATGGGCTGACGAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTGAATGGGAGCTGACGAAGTACAAAAAAAAAA30.14285714285714285No Hit
GTGAATGGGGAGCTGAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GAGTGAATGGGGCTGACAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GACGAAGTACGTACTCAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GAGCTGACGAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTCTGCGCTCTTTGGACTTTGAAAACTTAGCGA30.14285714285714285No Hit
GCTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
TATCAACGCAGAGTGAATGGGGAGTACGTACTT30.14285714285714285No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTGAATGGGGCTGACAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA30.14285714285714285No Hit
GTGAATGGGGCTGACGAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTACGTACTCTGCGTAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
ACGCAGAGTGAATGGGGCTGACAAAAAAAAAAA30.14285714285714285No Hit
GTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGG400.008520761516.8753
GTGAATG400.008520761516.8751
TGAATGG400.008520761516.8752
TCTGCGT600.004746545613.50000127
Produced by FastQC (version 0.11.2)