FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_37.3520000005fdb8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_37.3520000005fdb8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2160
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGTACTAGAGACGTACTCTGCGTTGATACCAC331.5277777777777777No Hit
GTACTAGAGAAAAAAAAAAAAAAAAAAAAAAAA301.3888888888888888No Hit
CTCTAGTACCGAAAAAAAAAAAAAAAAAAAAAA281.2962962962962963No Hit
GTACTAGAGACGTACTCAAAAAAAAAAAAAAAA241.1111111111111112No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.9722222222222222No Hit
GTACTAGAGACAAAAAAAAAAAAAAAAAAAAAA150.6944444444444444No Hit
GTACTAGAGACGTAAAAAAAAAAAAAAAAAAAA120.5555555555555556No Hit
GTGAATGGGTCGGTACAAAAAAAAAAAAAAAAA100.4629629629629629No Hit
GTACTAGAAAAAAAAAAAAAAAAAAAAAAAAAA100.4629629629629629No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.4166666666666667No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2777777777777778No Hit
GTGAATGGGATCGGTACAAAAAAAAAAAAAAAA60.2777777777777778No Hit
GTACTAGAGACGAAAAAAAAAAAAAAAAAAAAA60.2777777777777778No Hit
CCTCGGTACTAGAGACGTACTCTGCGTTGATAC60.2777777777777778No Hit
GTACTAGAGACGTACAAAAAAAAAAAAAAAAAA60.2777777777777778No Hit
GGTCGGTACTAGAGAAAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
CTCTAGTACCAAAAAAAAAAAAAAAAAAAAAAA50.23148148148148145No Hit
CGGTACTAGAGACGTACTCTGCGTTTATACCAC50.23148148148148145No Hit
GTGAATGGGTCGGTACTAGAGAAAAAAAAAAAA50.23148148148148145No Hit
ACGCAGAGTGAATGGGGTCGGTACAAAAAAAAA50.23148148148148145No Hit
ACTCGGTACTAGAGACGTACTCTGCGTTGATAC50.23148148148148145No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGTACTAGAGACGT40.1851851851851852No Hit
ACGCAGAGTGAATGGGATCGGTACAAAAAAAAA40.1851851851851852No Hit
ACGCAGAGTGAATGGGTACTAGAGACAAAAAAA40.1851851851851852No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA40.1851851851851852No Hit
AACGCAGAGTGAATGGGTACTAGAGACGTACTC40.1851851851851852No Hit
CTCTTTGGACTTTGAAAACTTAGCGAACTGAAA40.1851851851851852No Hit
TATCAACGCAGAGTGAATGGGATCGGTACAAAA40.1851851851851852No Hit
GAGTGAATGGGATCGGTACAAAAAAAAAAAAAA40.1851851851851852No Hit
GGTCGGTACAAAAAAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GGTCGGTACTAGAGACGTACTCTGCGTTGATAC40.1851851851851852No Hit
CTAGAGACGTACTCAAAAAAAAAAAAAAAAAAA40.1851851851851852No Hit
GTATCAACGCAGAGTGAATGGGATCGGTACTAG30.1388888888888889No Hit
GAATGGGTCGGTACAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGCTCGGTACTAGA30.1388888888888889No Hit
GTATCAACGCAGAGTGAATGGGTACTAGAGACG30.1388888888888889No Hit
GTGAATGGGTACTAGAGACGAAAAAAAAAAAAA30.1388888888888889No Hit
GTGAATGGGGTCGGTACAAAAAAAAAAAAAAAA30.1388888888888889No Hit
ACGCAGAGTGAATGGGTCGGTACTAAAAAAAAA30.1388888888888889No Hit
GATCGGTACAAAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GGTATCAACGCAGAGTGAATGGGTCGGTACAAA30.1388888888888889No Hit
GTGAATGGGTACTAGAGACGTACAAAAAAAAAA30.1388888888888889No Hit
CGGTACTAGAGAAAAAAAAAAAAAAAAAAAAAA30.1388888888888889No Hit
GTGAATGGGATCGGTACTAGAGAAAAAAAAAAA30.1388888888888889No Hit
CTTCGGTACTAGAGACGTACTCTGCGTTGATAC30.1388888888888889No Hit
GAGTGAATGGGTACTAGAGACAAAAAAAAAAAA30.1388888888888889No Hit
GATCGGTACTAGAGACGTACTCTGCGTTGATAC30.1388888888888889No Hit
ACGCAGAGTGAATGGGTCGGTACAAAAAAAAAA30.1388888888888889No Hit
GAGTGAATGGGATCGGTACTAGAGAAAAAAAAA30.1388888888888889No Hit
TATCAACGCAGAGTGAATGGGTCGGTACTAGAG30.1388888888888889No Hit
GTCGGTACTAGAGACGTACTCTGCGTTGATACC30.1388888888888889No Hit
GAATGGGGAGTACGTCTCTAGTACCGAAAAAAA30.1388888888888889No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCAGA456.954537E-418.01
GCAGAGT456.954537E-418.03
CGCAGAG456.954537E-418.02
CAGAGTG400.00859154516.8754
AGAGTGA400.00859154516.8755
Produced by FastQC (version 0.11.2)