FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_35.3520000005fd75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_35.3520000005fd75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7740
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTCTGTGACGTACTCTGCGTTGATACCAC1061.3695090439276485No Hit
GTCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA390.5038759689922481No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.31007751937984496No Hit
GTCCTAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.2583979328165375No Hit
CAGTCCTTCTGTGACGTACTCTGCGTTGATACC190.24547803617571057No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA170.21963824289405687No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA120.15503875968992248No Hit
GAGTCCTTCTGTGACGTACTCTGCGTTGATACC120.15503875968992248No Hit
GTCCTTCTGTGACGTACTCTGCGTTTATACCAC110.1421188630490956No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA90.11627906976744186No Hit
CCCTTAGTCCTTCTGTGACGTACTCTGCGTTGA80.10335917312661498No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCAGTC350.005059066719.2857158
TCTGCGT2100.013.527
GCGTTGA700.001140806713.527
CTCTGCG2550.011.11764626
ACTCTGC2901.0913936E-119.77586225
TACTCTG3002.0008883E-119.4524
AGTCCTT3207.4578566E-118.8593759
CGTACTC3053.2014214E-108.85245922
GTACTCT3104.3837645E-108.70967723
TCCTTCT3301.4006218E-108.59090811
GTCCTTC3301.4006218E-108.59090810
CCTTCTG3301.4006218E-108.59090812
ACGTACT3155.9299055E-108.57142821
TTCTGTG3351.8735591E-108.46268714
GACGTAC3208.0035534E-108.437520
TCTGTGA3402.5283953E-108.33823515
CTGTGAC3402.5283953E-108.33823516
CTTCTGT3402.5283953E-108.33823513
TGACGTA3251.0750227E-98.30769219
TGTGACG3301.4370016E-98.18181717
Produced by FastQC (version 0.11.2)