FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_34.3520000005fd5c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_34.3520000005fd5c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19325
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGAGACGAGTACGTACTCTGCGTTGATACCA2121.0970245795601552No Hit
CATCGAGACGAGTACGTACTCTGCGTTGATACC390.2018111254851229No Hit
CGAGACGAGTACGTACTCTGCGTTGATACCACT290.1500646830530401No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA280.1448900388098318No Hit
GATCGAGACGAGTACGTACTCTGCGTTGATACC270.13971539456662355No Hit
GTATCGAGACGAGTACGTACTCTGCGTTGATAC230.11901681759379043No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC230.11901681759379043No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1851.4188117E-1012.41378127
CATCGAG1402.0945969E-49.6493678
CTCTGCG2502.0816515E-89.18619826
ACTCTGC2759.793257E-88.351089525
TACTCTG2953.0319097E-77.784913524
GTACTCT3055.1678217E-77.529670223
ACGTACT3352.8453724E-77.25862921
GTACGTA3352.8453724E-77.25862919
AGTACGT3403.6464917E-77.151883618
TACGTAC3403.6464917E-77.151883620
GAGTACG3403.6464917E-77.151883617
AGACGAG3403.812056E-77.133307513
CGAGTAC3403.812056E-77.133307516
CGTACTC3251.418397E-67.06630622
CGAGACG3454.864869E-77.029926311
GAGACGA3454.864869E-77.029926312
ACGAGTA3506.183709E-76.929499615
GACGAGT3506.183709E-76.929499614
TCGAGAC3557.4916534E-76.849691410
ATCGAGA3701.4877696E-66.57200159
Produced by FastQC (version 0.11.2)