FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_33.3520000005fd32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_33.3520000005fd32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2040
Sequences flagged as poor quality0
Sequence length33
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGCCATTCCGTTACGTACTCTGCGTTGATACCA140.6862745098039216No Hit
GCCATTCCGTTACGTACTCTGCGTTGATACCAC120.5882352941176471No Hit
CAGCCATTCCGTTACGTACTCTGCGTTGATACC100.49019607843137253No Hit
CCATTCCGTTACGTACTCTGCGTTGATACCACT100.49019607843137253No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA90.4411764705882353No Hit
GAGCCATTCCGTTACGTACTCTGCGTTGATACC80.39215686274509803No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA70.3431372549019608No Hit
TAGCCATTCCGTTACGTACTCTGCGTTGATACC70.3431372549019608No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA60.29411764705882354No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA50.24509803921568626No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA50.24509803921568626No Hit
GTACGTAACGGAAAAAAAAAAAAAAAAAAAAAA50.24509803921568626No Hit
GCCATTCCGTTACGTACTCTGCGTTTATACCAC50.24509803921568626No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.24509803921568626No Hit
GTGAATGGGGAGTACGTAACGAAAAAAAAAAAA50.24509803921568626No Hit
GCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
ACGCAGAGTGAATGGGGAGTACGTAACGAAAAA40.19607843137254902No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
GAGTACGTAACGAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
GTACGTAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19607843137254902No Hit
CCCCCAGCCATTCCGTTACGTACTCTGCGTTGA30.14705882352941177No Hit
GTGAATGGGGTACGTAACGAAAAAAAAAAAAAA30.14705882352941177No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA30.14705882352941177No Hit
ATCAACGCAGAGTGAATGGGGAGTACGTAACGA30.14705882352941177No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.2)