FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_32.3520000005fd25.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_32.3520000005fd25.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4560
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTGAATGGGCTAACTATCCAC611.337719298245614No Hit
CTATCCACGTACTCAAAAAAAAAAAAAAAAAAA591.2938596491228072No Hit
GTGAATGGGCTAACTATCCACAAAAAAAAAAAA581.2719298245614035No Hit
GGCTAACTATCCACGTACTCTGCGTTGATACCA501.0964912280701753No Hit
ACGCAGAGTGAATGGGCTAACTATCCACAAAAA390.855263157894737No Hit
GTATCAACGCAGAGTGAATGGGCTAACTATCCA330.7236842105263158No Hit
GTGAATGGGGCTAACTATCCACAAAAAAAAAAA280.6140350877192983No Hit
GGCTAACTATCCACAAAAAAAAAAAAAAAAAAA260.5701754385964912No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.5263157894736842No Hit
ATCCACGTACTCAAAAAAAAAAAAAAAAAAAAA240.5263157894736842No Hit
AACGCAGAGTGAATGGGCTAACTATCCACAAAA230.5043859649122807No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACT230.5043859649122807No Hit
GAGTGAATGGGCTAACTATCCACAAAAAAAAAA220.4824561403508772No Hit
ATCCACAAAAAAAAAAAAAAAAAAAAAAAAAAA220.4824561403508772No Hit
AACGCAGAGTGAATGGGCTAACTATCCACGTAC200.43859649122807015No Hit
GCTAACTATCCACGTACTCTGCGTTGATACCAC200.43859649122807015No Hit
GTGAATGGGCTAACTATCCAAAAAAAAAAAAAA170.37280701754385964No Hit
ACTATCCACAAAAAAAAAAAAAAAAAAAAAAAA170.37280701754385964No Hit
ATCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA160.3508771929824561No Hit
TATCAACGCAGAGTGAATGGGGCTAACTATCCA160.3508771929824561No Hit
GTGAATGGGCTAACTATCCACGTACAAAAAAAA160.3508771929824561No Hit
GGCTAACTATCCACGTACTCAAAAAAAAAAAAA150.3289473684210526No Hit
CTATCCACAAAAAAAAAAAAAAAAAAAAAAAAA140.30701754385964913No Hit
GTGAATGGGCTAACTAAAAAAAAAAAAAAAAAA140.30701754385964913No Hit
GTGAATGGGCTAACTATCCACGTACTCAAAAAA140.30701754385964913No Hit
ACGCAGAGTGAATGGGCTAACTATCCAAAAAAA130.2850877192982456No Hit
GAGTGAATGGGCTAACTATCCACGTACTCAAAA120.2631578947368421No Hit
GGTATCAACGCAGAGTGAATGGGCTAACTATCC120.2631578947368421No Hit
TATCAACGCAGAGTGAATGGGCTAACTATCCAA120.2631578947368421No Hit
TATCAACGCAGAGTGAATGGGCTAACTAAAAAA110.2412280701754386No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACGTAC110.2412280701754386No Hit
CGTACTCAAAAAAAAAAAAAAAAAAAAAAAAAA110.2412280701754386No Hit
GAGTGAATGGGGCTAACTATCCACGTACTCAAA110.2412280701754386No Hit
CTATAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.21929824561403508No Hit
GTATCAACGCAGAGTGAATGGGGCTAACTATCC100.21929824561403508No Hit
GGGCTAACTATCCACAAAAAAAAAAAAAAAAAA90.19736842105263158No Hit
GAATGGGCTAACTATCCACGTACTCAAAAAAAA90.19736842105263158No Hit
CTAACTATCCACGTACTCTGCGTTGATACCACT90.19736842105263158No Hit
ATCCACGTACAAAAAAAAAAAAAAAAAAAAAAA90.19736842105263158No Hit
ACTATCCACGTACTCAAAAAAAAAAAAAAAAAA90.19736842105263158No Hit
GTGAATGGGGCTAACTATCCACGTACTCAAAAA90.19736842105263158No Hit
CTATCCACGTACAAAAAAAAAAAAAAAAAAAAA90.19736842105263158No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA90.19736842105263158No Hit
AACGCAGAGTGAATGGGCTAACTATAAAAAAAA80.17543859649122806No Hit
CAACGCAGAGTGAATGGGCTAACTATCCACAAA80.17543859649122806No Hit
TATCAACGCAGAGTGAATGGGGCTAACTAAAAA80.17543859649122806No Hit
GAGTGAATGGGCTAACTATCCACGTACAAAAAA80.17543859649122806No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACA80.17543859649122806No Hit
GAGTGAATGGGGCTAACTAAAAAAAAAAAAAAA80.17543859649122806No Hit
GAGTGAATGGGGCTAACTATCCACAAAAAAAAA80.17543859649122806No Hit
GAGTGAATGGGCTAACTATCCAAAAAAAAAAAA80.17543859649122806No Hit
CGTACAAAAAAAAAAAAAAAAAAAAAAAAAAAA80.17543859649122806No Hit
CTATCCAAAAAAAAAAAAAAAAAAAAAAAAAAA70.15350877192982457No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACAAA70.15350877192982457No Hit
GCTAACTATCCACGTACTCAAAAAAAAAAAAAA70.15350877192982457No Hit
CACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA70.15350877192982457No Hit
GTGAATGGGGCTAACTAAAAAAAAAAAAAAAAA70.15350877192982457No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA60.13157894736842105No Hit
ACGCAGAGTGAATGGGCTAACTATCCACGTACA60.13157894736842105No Hit
GAGTGAATGGGCTAACTAAAAAAAAAAAAAAAA60.13157894736842105No Hit
GTGAATGGGCTAACTATCAAAAAAAAAAAAAAA60.13157894736842105No Hit
AACGCAGAGTGAATGGGCTAACTATCCAAAAAA60.13157894736842105No Hit
ATCAACGCAGAGTGAATGGGCTAACTATCCACG60.13157894736842105No Hit
GCTAACTATCCACAAAAAAAAAAAAAAAAAAAA60.13157894736842105No Hit
AACGCAGAGTGAATGGGGCTAACTATCCACGTA60.13157894736842105No Hit
GGCTAACTATCCAAAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
GTGAATGGGGCTAACTATCCACGTACAAAAAAA50.10964912280701754No Hit
ACGCAGAGTGAATGGGGCTAACTATCCACAAAA50.10964912280701754No Hit
GTATCATCGCAGAGTGAATGGGCTAACTATCCA50.10964912280701754No Hit
GTGAATGGGGCTAACAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
TATCTACGCAGAGTGAATGGGGCTAACTATCCA50.10964912280701754No Hit
GGGCTAACTATCCACGTACTCTGCGTTGATACC50.10964912280701754No Hit
TCCACGTACTCAAAAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
GTGAATGGGCTAACTATCCACGTACTCTGCGTT50.10964912280701754No Hit
GAGTAGGAGGGCGCGGCGGTCGCTGCAAAACCT50.10964912280701754No Hit
CGGCTAACTATCCACGTACTCTGCGTTGATACC50.10964912280701754No Hit
GTGAATGGGCTAACAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
TCCACGTACAAAAAAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
GAATGGGCTAACTATCCACAAAAAAAAAAAAAA50.10964912280701754No Hit
CTAACTATCCACAAAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
ACGCAGAGTGAATGGGCTAACTATAAAAAAAAA50.10964912280701754No Hit
ACTATCCACGTACAAAAAAAAAAAAAAAAAAAA50.10964912280701754No Hit
ATGGCTAACTATCCACGTACTCTGCGTTGATAC50.10964912280701754No Hit
GAATGGGGCTAACTATCCACGTACTCAAAAAAA50.10964912280701754No Hit
CCGGCTAACTATCCACGTACTCTGCGTTGATAC50.10964912280701754No Hit
ACGCAGAGTGAATGGGGCTAACTAAAAAAAAAA50.10964912280701754No Hit
GTCTACGAGTCGGGTTGTTTGGGAATGCAGCCC50.10964912280701754No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCAACG458.9656777E-418.02
TCAACGC500.00181932216.23
TATCAAC500.00181932216.21
CAACGCA500.00181932216.24
TCTGCGT954.553394E-512.78947427
CTCTGCG1152.7283165E-410.56521826
ACTCTGC1308.4957224E-49.34615425
GTACTCT1350.001202419.023
TACTCTG1350.001202419.024
TGAATGG1400.00167857948.6785722
GTGAATG1450.00231359398.3793111
TCCACGT1750.00133320377.714285418
GAATGGG1600.0056576747.593753
CGTACTC1600.0056576747.5937522
AACTATC2151.2700586E-47.534883513
ACTATCC2151.2700586E-47.534883514
CCACGTA1800.00176378237.519
TATCCAC2005.4415676E-47.42516
CACGTAC1850.00231368517.29729720
CTATCCA2252.1609759E-47.215
Produced by FastQC (version 0.11.2)