FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_30.3520000005fcd3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_30.3520000005fcd3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3300
Sequences flagged as poor quality0
Sequence length33
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCGTCACACTACGTACTCTGCGTTGATACCA421.2727272727272727No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA240.7272727272727273No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA230.6969696969696969No Hit
GTGAATGGGTTCGTCACAAAAAAAAAAAAAAAA180.5454545454545455No Hit
GTGAATGGGTTCGTCAAAAAAAAAAAAAAAAAA150.45454545454545453No Hit
GAGTGAATGGGAAGCAGTGGTATCAAAAAAAAA130.393939393939394No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA130.393939393939394No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA120.36363636363636365No Hit
ACGCAGAGTGAATGGGAAGCAGTGGTATCAAAA120.36363636363636365No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.33333333333333337No Hit
TCACACTACGTACTCAAAAAAAAAAAAAAAAAA100.30303030303030304No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.30303030303030304No Hit
CTACGTACTCAAAAAAAAAAAAAAAAAAAAAAA90.27272727272727276No Hit
GTGAATGGGTTCGTCACACAAAAAAAAAAAAAA70.21212121212121215No Hit
GTGAATGGGGTTCGTCACACTACGTACTCAAAA70.21212121212121215No Hit
GTGAATGGGTTCGTCACACTACGTACTCAAAAA70.21212121212121215No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA70.21212121212121215No Hit
TATCAACGCAGAGTGAATGGGGTTCGTCACACA70.21212121212121215No Hit
GTCACACTACGTACTCAAAAAAAAAAAAAAAAA70.21212121212121215No Hit
AACGCAGAGTGAATGGGTTCGTCACAAAAAAAA60.18181818181818182No Hit
TCGTCACACTACGTACTCAAAAAAAAAAAAAAA60.18181818181818182No Hit
GTGAATGGGGTTCGTCAAAAAAAAAAAAAAAAA60.18181818181818182No Hit
ACGCAGAGTGAATGGGGTTCGTCAAAAAAAAAA60.18181818181818182No Hit
TATCAACGCAGAGTGAATGGGTTCGTCACACTA60.18181818181818182No Hit
TTCGTCACACTACGTACTCTGCGTTGATACCAC60.18181818181818182No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT50.15151515151515152No Hit
GTACGTAGTGTGACAAAAAAAAAAAAAAAAAAA50.15151515151515152No Hit
GTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA50.15151515151515152No Hit
GTGAATGGGGTTCGTCACAAAAAAAAAAAAAAA50.15151515151515152No Hit
ACGCAGAGTGAATGGGTTCGTCAAAAAAAAAAA50.15151515151515152No Hit
GTCACACTACGTACAAAAAAAAAAAAAAAAAAA50.15151515151515152No Hit
GGTATCAACGCAGAGTGAATGGGAAGCAGTGGT40.12121212121212122No Hit
GAGTGAATGGGGTTCGTCAAAAAAAAAAAAAAA40.12121212121212122No Hit
ACGCAGAGTGAATGGGTTCGTCACACTACGTAC40.12121212121212122No Hit
GTGTTCGTCACACTACGTACTCTGCGTTGATAC40.12121212121212122No Hit
GTTCGTCACACTACAAAAAAAAAAAAAAAAAAA40.12121212121212122No Hit
CTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.12121212121212122No Hit
GAGTGAATGGGTTCGTCACACAAAAAAAAAAAA40.12121212121212122No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA40.12121212121212122No Hit
GGGTTCGTCACACTACGTACTCTGCGTTGATAC40.12121212121212122No Hit
CTACGTACTCTGCGTTGATACCAAAAAAAAAAA40.12121212121212122No Hit
CCTCCGTTCGTCACACTACGTACTCTGCGTTGA40.12121212121212122No Hit
GAATGGGAAGCAGTGGTATCAAAAAAAAAAAAA40.12121212121212122No Hit
ATCAACGCAGAGTGAATGGGAAGCAGTGGTATC40.12121212121212122No Hit
GTATCAACGCAGAGTGAATGGGTTCGTCACACT40.12121212121212122No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA300.001824199622.51
TATCAAC300.001824199622.52
ATCAACG350.004469197219.2857153
TCTGCGT751.7287493E-718.027
TCAACGC400.0096751916.8754
CTCTGCG952.0778207E-614.21052626
ACTCTGC1202.3539389E-511.2525
TACTCTG1305.3630756E-510.38461524
GTACTCT1357.89443E-510.023
ACGTACT1451.6364791E-49.31034621
CGTACTC1451.6364791E-49.31034622
TACGTAC1553.218921E-48.70967720
CTACGTA1604.4358397E-48.437519
CACTACG1656.047415E-48.18181817
ACTACGT1656.047415E-48.18181818
CGTCACA1800.00144542037.512
CACACTA1800.00144542037.515
ACACTAC1800.00144542037.516
TCGTCAC1800.00144542037.511
TCACACT1850.0018987247.29729814
Produced by FastQC (version 0.11.2)