FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_3.3520000005f986.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_3.3520000005f986.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2100
Sequences flagged as poor quality0
Sequence length33
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTGTTCCTCACGTACTCTGCGTTGATACCA562.666666666666667No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.4761904761904762No Hit
CTCTGTTCCTCACGTACTCTGCGTTGATACCAC100.4761904761904762No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA70.33333333333333337No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA60.2857142857142857No Hit
GTTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.2857142857142857No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA60.2857142857142857No Hit
TATCAACGCAGAGTGAATGGGAAGCAGTGGTAT50.2380952380952381No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA50.2380952380952381No Hit
GTGAATGGGACTCTGTTCCAAAAAAAAAAAAAA50.2380952380952381No Hit
GAATGGGACTCTGTTCAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
CTACTCTGTTCCTCACGTACTCTGCGTTGATAC40.19047619047619047No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA40.19047619047619047No Hit
GTATCAACGCAGAGTGAATGGGACTCTGTAAAA30.14285714285714285No Hit
ACTCTGTTCCTCAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
CCCCTACTCTGTTCCTCACGTACTCTGCGTTGA30.14285714285714285No Hit
CCGCCACTCTGTTCCTCACGTACTCTGCGTTGA30.14285714285714285No Hit
GGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit
GTCTTACTCTGTTCCTCACGTACTCTGCGTTGA30.14285714285714285No Hit
GTGAATGGGGACTCTGTAAAAAAAAAAAAAAAA30.14285714285714285No Hit
CCCTCACTCTGTTCCTCACGTACTCTGCGTTGA30.14285714285714285No Hit
GTGAATGGGACTCTGTTCAAAAAAAAAAAAAAA30.14285714285714285No Hit
GAGTGAATGGGACTCTGTTCAAAAAAAAAAAAA30.14285714285714285No Hit
GGGCAGACTCAAAAAAAAAAAAAAAAAAAAAAA30.14285714285714285No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT955.5590135E-815.631577527
CTCTGCG1208.1037433E-712.37500126
CACGTAC1208.1037433E-712.37500120
ACGTACT1208.1037433E-712.37500121
ACTCTGC1208.1037433E-712.37500125
TCACGTA1208.1037433E-712.37500119
CGTACTC1208.1037433E-712.37500122
CTCACGT1208.1037433E-712.37500118
CTCTGTT1251.2901764E-611.8810
TCCTCAC1159.376476E-611.73913116
CCTCACG1159.376476E-611.73913117
GTACTCT1302.0149364E-611.42307723
ACTCTGT1302.0149364E-611.4230779
GTTCCTC1201.4598594E-511.25000114
TGTTCCT1201.4598594E-511.25000113
TCTGTTC1201.4598594E-511.25000111
TTCCTCA1201.4598594E-511.25000115
CTGTTCC1201.4598594E-511.25000112
TACTCTG1404.6652967E-610.60714324
Produced by FastQC (version 0.11.2)