FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_27.3520000005fc76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_27.3520000005fc76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200.49019607843137253No Hit
TCGTTGGTCGAAACGTACTCTGCGTTGATACCA140.3431372549019608No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA100.24509803921568626No Hit
GTGAATGGGGAGTACGTTTCGACCAAAAAAAAA80.19607843137254902No Hit
GTTTCGACCAAAAAAAAAAAAAAAAAAAAAAAA80.19607843137254902No Hit
CAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAA70.1715686274509804No Hit
GCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAA70.1715686274509804No Hit
GTATCAACGCAGAGTGAATGGGAAGCAGTGGTA70.1715686274509804No Hit
CTCGTTGGTCGAAACGTACTCTGCGTTGATACC70.1715686274509804No Hit
GTGAATGGGTACGTTTCGACCAAAAAAAAAAAA50.12254901960784313No Hit
GTGAATGGGAAGCAGTGGTATCAAAAAAAAAAA50.12254901960784313No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA50.12254901960784313No Hit
CGTTTCGACCAAAAAAAAAAAAAAAAAAAAAAA50.12254901960784313No Hit
AACGCAGAGTGAATGGGAAGCAGTGGTATCAAA50.12254901960784313No Hit
GTGAATGGGTCGTTGGTAAAAAAAAAAAAAAAA50.12254901960784313No Hit
GTGAATGGGTCGTTGGAAAAAAAAAAAAAAAAA50.12254901960784313No Hit
GTGAATGGGTCGTTGGTCAAAAAAAAAAAAAAA50.12254901960784313No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATGGG600.0059807113.53
Produced by FastQC (version 0.11.2)