FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_23.3520000005fc0c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_23.3520000005fc0c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9481
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGCAGATACGTACTCTGCGTTGATACCAC951.002004008016032No Hit
GCAGATACGTACTCAAAAAAAAAAAAAAAAAAA860.9070773125197764No Hit
TATCAACGCAGAGTGAATGGGATAGCAGATACG580.6117498154203144No Hit
GATACGTACTCAAAAAAAAAAAAAAAAAAAAAA520.5484653517561439No Hit
GTGAATGGGATAGCAGATACGTACTCAAAAAAA510.5379179411454488No Hit
GTGAATGGGATAGCAGATACGTACAAAAAAAAA420.4429912456491931No Hit
GCAGATACGTACAAAAAAAAAAAAAAAAAAAAA340.35861196076363255No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA330.34806455015293747No Hit
GATAGCAGATACGTACTCTGCGTTGATACCACT310.3269697289315473No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACTC240.25313785465668176No Hit
GTATCAACGCAGAGTGAATGGGATAGCAGATAC230.2425904440459867No Hit
AACGCAGAGTGAATGGGATAGCAGATACGTACT220.23204303343529165No Hit
TATCTACGCAGAGTGAATGGGATAGCAGATACG210.22149562282459656No Hit
GTGAATGGGATAGCAGATACGTACTAAAAAAAA190.2004008016032064No Hit
CCGGATAGCAGATACGTACTCTGCGTTGATACC170.17930598038181628No Hit
GTACTCAAAAAAAAAAAAAAAAAAAAAAAAAAA160.1687585697711212No Hit
ACGGATAGCAGATACGTACTCTGCGTTGATACC150.15821115916042613No Hit
GAGTGAATGGGATAGCAGATACGTACTCAAAAA150.15821115916042613No Hit
GGATAGCAGATACGTACAAAAAAAAAAAAAAAA150.15821115916042613No Hit
ACGCAGAGTGAATGGGATAGCAGATACGTACAA140.14766374854973105No Hit
GGATAGCAGATACGTACTCAAAAAAAAAAAAAA140.14766374854973105No Hit
GTACTAAAAAAAAAAAAAAAAAAAAAAAAAAAA140.14766374854973105No Hit
GATACGTACTAAAAAAAAAAAAAAAAAAAAAAA130.13711633793903596No Hit
TACGTACTCAAAAAAAAAAAAAAAAAAAAAAAA130.13711633793903596No Hit
GGTATCAACGCAGAGTGAATGGGATAGCAGATA130.13711633793903596No Hit
CAACTCAGAGTGAATGGGATAGCAGATACGTAC130.13711633793903596No Hit
ATCAACGCAGAGTGAATGGGATAGCAGATACGT120.12656892732834088No Hit
GTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAA120.12656892732834088No Hit
GCAGATACGTACTAAAAAAAAAAAAAAAAAAAA120.12656892732834088No Hit
GAGTGAATGGGATAGCAGATACGTACAAAAAAA110.11602151671764582No Hit
GTACAAAAAAAAAAAAAAAAAAAAAAAAAAAAA110.11602151671764582No Hit
CAACGCAGAGTGAATGGGATAGCAGATACGTAC110.11602151671764582No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA110.11602151671764582No Hit
GATACGTACAAAAAAAAAAAAAAAAAAAAAAAA100.10547410610695074No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC100.10547410610695074No Hit
GTGAATGGGCGGATAGCAAAAAAAAAAAAAAAA100.10547410610695074No Hit
GTATCATCGCAGAGTGAATGGGATAGCAGATAC100.10547410610695074No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA200.00819278427.023
GATACCA200.00819278427.026
TGATACC200.00819278427.025
TTGATAC200.00819278427.024
ATACCAC200.00819278427.027
TATCAAC509.036703E-518.91
TCTGCGT901.0372787E-716.50000227
TCAACGC500.002033851316.1999993
ATCAACG500.002033851316.1999992
CCGGATA750.001979138212.68
CTCTGCG1351.0090267E-511.00000126
ACTCTGC1503.2456643E-59.90000125
TACTCTG1554.6590103E-59.58064524
GTACTCT1751.75871E-48.48571423
GTGAATG1700.00130032047.9411771
TGAATGG1700.00130032047.9411772
CGTACTC2101.4030284E-47.714286322
CGGATAG2059.6925534E-47.24390279
ACGTACT2355.1588146E-46.89361721
TACGTAC2600.00163598996.23076920
Produced by FastQC (version 0.11.2)