FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_20.3520000005fb91.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_20.3520000005fb91.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5401
Sequences flagged as poor quality0
Sequence length33
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGGAGACTACGTACTCTGCGTTGATACCACT1051.9440844288094796No Hit
ACGTAGGAGACTACGTACTCTGCGTTGATACCA711.314571375671172No Hit
ACGTAGGAGAAAAAAAAAAAAAAAAAAAAAAAA280.5184225143491945No Hit
GTGAATGGGACGTAGGAGAAAAAAAAAAAAAAA270.49990742455100906No Hit
ACGTAGGAGACAAAAAAAAAAAAAAAAAAAAAA250.462877244954638No Hit
GTGAATGGGACGTAGGAGACAAAAAAAAAAAAA230.425847065358267No Hit
GGACGTAGGAGACAAAAAAAAAAAAAAAAAAAA220.40733197556008144No Hit
ACGCAGAGTGAATGGGACGTAGGAGAAAAAAAA220.40733197556008144No Hit
GAGTGAATGGGACGTAGGAGACAAAAAAAAAAA190.35178670616552493No Hit
CGTAGGAGACTACGTACTCTGCGTTGATACCAC180.3332716163673394No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.2777263469727828No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGAAA140.25921125717459725No Hit
ACGCAGAGTGAATGGGACGTAGGAGACAAAAAA140.25921125717459725No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAA120.22218107757822625No Hit
GAACGTAGGAGACAAAAAAAAAAAAAAAAAAAA110.20366598778004072No Hit
GTAGGAGACAAAAAAAAAAAAAAAAAAAAAAAA100.18515089798185522No Hit
GGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100.18515089798185522No Hit
TATCAACGCAGAGTGAATGGGACGTAGGAGACA100.18515089798185522No Hit
GAGTGAATGGGACGTAGGAGAAAAAAAAAAAAA90.1666358081836697No Hit
CTACGTAGGAGACTACGTACTCTGCGTTGATAC90.1666358081836697No Hit
AACGCAGAGTGAATGGGACGTAGGAGACAAAAA90.1666358081836697No Hit
GAATGGGACGTAGGAGAAAAAAAAAAAAAAAAA80.14812071838548418No Hit
GAATGGGACGTAGGAGACAAAAAAAAAAAAAAA80.14812071838548418No Hit
GACGTAGGAGACAAAAAAAAAAAAAAAAAAAAA80.14812071838548418No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGAAAA70.12960562858729863No Hit
GCACGTAGGAGACAAAAAAAAAAAAAAAAAAAA70.12960562858729863No Hit
GTATCAACGCAGAGTGAATGGGACGTAGGAGAC70.12960562858729863No Hit
CACGTAGGAGACTACGTACTCTGCGTTGATACC70.12960562858729863No Hit
GCACGTAGGAGACTACGTACTCTGCGTTGATAC70.12960562858729863No Hit
ACGCAGAGTGAATGGGACGTAGGAAAAAAAAAA70.12960562858729863No Hit
GGACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA70.12960562858729863No Hit
CCACGTAGGAGACTACGTACTCTGCGTTGATAC70.12960562858729863No Hit
GTGAATGGGACGTAGGAAAAAAAAAAAAAAAAA70.12960562858729863No Hit
AAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAA60.11109053878911312No Hit
ATCAACGCAGAGTGAATGGGACGTAGGAGACAA60.11109053878911312No Hit
AACGCAGAGTGAATGGGACGTAGGAGAAAAAAA60.11109053878911312No Hit
GGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60.11109053878911312No Hit
GTGAATGGGGACGTAGGAGAAAAAAAAAAAAAA60.11109053878911312No Hit
GTGAATGGGAACGTAGGAGAAAAAAAAAAAAAA60.11109053878911312No Hit
GAACGTAGGAGAAAAAAAAAAAAAAAAAAAAAA60.11109053878911312No Hit
CCCCCACGTAGGAGACTACGTACTCTGCGTTGA60.11109053878911312No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCACT355.1384595E-627.027
ATACCAC401.4505675E-523.62526
GATACCA551.6915123E-417.18181825
TGATACC603.2912273E-415.75000124
TTGATAC603.2912273E-415.75000123
GTTGATA656.056625E-414.53846122
CGTTGAT656.056625E-414.53846121
ACGCAGA700.001062882213.51
CGCAGAG800.002910578411.81252
GCGTTGA800.002910578411.812520
TGCGTTG1256.338591E-49.72000127
CTGCGTT1309.100045E-49.34615326
TGAATGG1350.00128725459.02
GTGAATG1450.00247422638.3793111
TCTGCGT1550.00453428137.838709425
CTCTGCG1600.0060409737.5937524
GTACTCT1800.00191147947.521
ACGTACT1800.00191147947.519
CGTACTC1800.00191147947.520
ACTCTGC1650.0079700677.363636523
Produced by FastQC (version 0.11.2)