FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_18.3520000005fb5e.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_18.3520000005fb5e.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14642
Sequences flagged as poor quality0
Sequence length33
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCCAATGCACGTACTCTGCGTTGATACCAC1390.949323862860265No Hit
GGTATCCAATGCACGTACTCTGCGTTGATACCA970.6624778035787461No Hit
CCGGTATCCAATGCACGTACTCTGCGTTGATAC630.43026908892227833No Hit
ACGGTATCCAATGCACGTACTCTGCGTTGATAC460.3141647315940445No Hit
GCGGTATCCAATGCACGTACTCTGCGTTGATAC410.2800163912033875No Hit
CAGGTATCCAATGCACGTACTCTGCGTTGATAC380.2595273869689933No Hit
GGGTATCCAATGCACGTACTCTGCGTTGATACC370.2526977188908619No Hit
CTGGTATCCAATGCACGTACTCTGCGTTGATAC370.2526977188908619No Hit
GTGAATGGGGTATCCAAAAAAAAAAAAAAAAAA360.2458680508127305No Hit
ATGGTATCCAATGCACGTACTCTGCGTTGATAC280.1912307061876793No Hit
GTGAATGGGTATCCAATGCACAAAAAAAAAAAA270.1844010381095479No Hit
GTGAATGGGTATCCAAAAAAAAAAAAAAAAAAA270.1844010381095479No Hit
GGGGTATCCAATGCACGTACTCTGCGTTGATAC250.17074170195328509No Hit
TCGGTATCCAATGCACGTACTCTGCGTTGATAC250.17074170195328509No Hit
GTGGTATCCAATGCACGTACTCTGCGTTGATAC250.17074170195328509No Hit
CGGTATCCAATGCACGTACTCTGCGTTGATACC240.16391203387515366No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA240.16391203387515366No Hit
ACCTATACCCGGCCGTCGGGGCAAGAGCCAGGC240.16391203387515366No Hit
CGGGTATCCAATGCACGTACTCTGCGTTGATAC220.15025269771889088No Hit
TATCCAATGCACGTACTCTGCGTTGATACCACT210.14342302964075945No Hit
GAGGTATCCAATGCACGTACTCTGCGTTGATAC180.12293402540636525No Hit
AAGGTATCCAATGCACGTACTCTGCGTTGATAC180.12293402540636525No Hit
AGGGTATCCAATGCACGTACTCTGCGTTGATAC170.11610435732823385No Hit
GTATCCAATGCACGTACTCTGCGTTTATACCAC160.10927468925010243No Hit
AGCCCGGGCGGAGCGGCCGTCGGTGCAGATCTT160.10927468925010243No Hit
TAGGTATCCAATGCACGTACTCTGCGTTGATAC160.10927468925010243No Hit
GTGCAGATCTTGGTGGTAGTAGCAAAAAAAAAA150.10244502117197105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATA1251.202352E-915.1226
GCGTTGA1251.202352E-915.1224
CGTTGAT1302.0954758E-914.53846125
TTGATAC1403.6743586E-1014.46428627
TGCGTTG1353.5724952E-914.023
GAATGGG600.007092471713.53
CTGCGTT1709.00709E-811.11764722
TCTGCGT2800.011.08928627
TGGTATC1350.00160901569.08
CTCTGCG3601.6370905E-118.62500126
ACTCTGC4001.5279511E-107.762525
TACTCTG4102.582965E-107.573170724
ACGTACT4401.4006218E-107.363636521
GTACTCT4255.5661076E-107.305882523
CGTACTC4451.7826096E-107.28089922
CACGTAC4502.2919266E-107.220
TGCACGT4603.7107384E-107.04347818
CAATGCA4654.7111826E-106.96774215
AATGCAC4501.8608262E-96.899999616
GCACGTA4705.9480953E-106.893616719
Produced by FastQC (version 0.11.2)