FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_17.3520000005fb34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_17.3520000005fb34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11041
Sequences flagged as poor quality0
Sequence length33
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CATACGCACGTTACGTACTCTGCGTTGATACCA1311.186486731274341No Hit
CCATACGCACGTTACGTACTCTGCGTTGATACC460.4166289285390816No Hit
ATACGCACGTTACGTACTCTGCGTTGATACCAC410.3713431754370075No Hit
CCCATACGCACGTTACGTACTCTGCGTTGATAC410.3713431754370075No Hit
GCATACGCACGTTACGTACTCTGCGTTGATACC390.3532288741961779No Hit
ACATACGCACGTTACGTACTCTGCGTTGATACC240.21737161488995563No Hit
CACATACGCACGTTACGTACTCTGCGTTGATAC180.1630287111674667No Hit
CTCATACGCACGTTACGTACTCTGCGTTGATAC160.1449144099266371No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA150.13585725930622225No Hit
CCCCATACGCACGTTACGTACTCTGCGTTGATA150.13585725930622225No Hit
GCCATACGCACGTTACGTACTCTGCGTTGATAC130.11774295806539262No Hit
GTCATACGCACGTTACGTACTCTGCGTTGATAC130.11774295806539262No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGCGT1600.00718857637.5937527
CTCTGCG1850.0030951117.29729726
Produced by FastQC (version 0.11.2)