FastQCFastQC Report
Thu 30 Jun 2016
HTC23BCXX_l02n02_kb_plate7_14.3520000005fad5.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHTC23BCXX_l02n02_kb_plate7_14.3520000005fad5.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17403
Sequences flagged as poor quality0
Sequence length33
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAACAGAGCATACGTACTCTGCGTTGATACCAC2831.6261564098144No Hit
GCAACAGAGCATACGTACTCTGCGTTGATACCA790.45394472217433773No Hit
GAGCATACGTACTCAAAAAAAAAAAAAAAAAAA500.2873067861862897No Hit
CCGCAACAGAGCATACGTACTCTGCGTTGATAC470.2700683790151123No Hit
CCCCGCAACAGAGCATACGTACTCTGCGTTGAT270.15514566454059645No Hit
CAACAGAGCATACGTACTCTGCGTTTATACCAC270.15514566454059645No Hit
GTCTTGTCGTGCGCGTTGCTTCCGGATATCACA260.14939952881687066No Hit
TATCAACGCAGAGTGAATGGGCAACAGAGCATA240.13790725736941908No Hit
GTGAATGGGCAACAGAGCAAAAAAAAAAAAAAA240.13790725736941908No Hit
GCGCAACAGAGCATACGTACTCTGCGTTGATAC230.13216112164569327No Hit
CTTTTATCTCGGTCTTGTCGTGCGCGTTGCTTC220.12641498592196748No Hit
GTATTGTAAGTGGCAGAGTGGCCTTGCTGCCAC220.12641498592196748No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA210.12066885019824168No Hit
ACGCAGAGTGAATGGGCAACAGAGCAAAAAAAA210.12066885019824168No Hit
CTGCAACAGAGCATACGTACTCTGCGTTGATAC200.11492271447451588No Hit
ACGCAACAGAGCATACGTACTCTGCGTTGATAC200.11492271447451588No Hit
TCACAAAACCCCGGCACGAAAAGTGTCAAGGAA190.1091765787507901No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCAC509.623232E-518.927
ATGGGCA450.001051673418.0000025
TGATACC601.8652356E-518.025
AATGGGC500.002130334716.1999994
GATACCA603.880883E-415.7526
GAATGGG850.005364807311.1176463
GTGAATG850.005364807311.1176461
TGAATGG850.005364807311.1176462
GTTGATA1000.001428893410.79999923
TTGATAC1000.001428893410.79999924
TCTGCGT2502.0791049E-78.6427
CTCTGCG2706.7347537E-78.026
GCAACAG3804.5747583E-97.46052559
ACTCTGC2901.9867766E-67.44827625
CGTACTC3454.5400884E-77.04347822
GTACTCT3301.7674356E-66.954545523
TACTCTG3156.862443E-66.857142424
ACGTACT3557.3102274E-76.845070421
CAACAGA4203.169407E-86.750000510
CAGAGCA4203.169407E-86.750000513
Produced by FastQC (version 0.11.2)